28个不同采集地土茯苓(断面红色、白色)中落新妇苷分析及抗炎作用的比较  

Comparison of anti-inflammatory effect and analysis of astilbin red and white transverse section Smilax glabra in 28 collection sites

作　　者：何席呈[1] 孙庆文[1] 董立莎[1] 葛向前[1] 张华[1] 农亨[1] 沈晓华[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《中国中药杂志》2012年第23期3595-3598,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30960503);贵州省科学技术基金项目(黔科合丁字[2008]2143号)

摘　　要：目的:采用HPLC分析测定28个不同采集地土茯苓(断面红色、白色)中落新妇苷的含量,并通过对土茯苓(断面红色、白色)渗透型炎症的研究,拟对该品种进行药效与成分的评价。方法:RP-HPLC测定落新妇苷含量,采用依利特Hy-persil ODS2色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%冰醋酸溶液(21∶79),流速1.0 mL.min-1,检测波长291 nm,柱温25℃,理论塔板数以落新妇苷计不得低于5 000。用毛细血管通透性试验比较不同断面颜色药的抗炎作用。结果:在选定的色谱条件下,落新妇苷在0.003 379～4.004μg呈良好线性关系。加样回收实验加样80%,100%,120%,平均回收率分别为100.1%,101.9%,99.3%。断面白色土茯苓中落新妇苷质量分数为0.19%～2.46%,断面红色土茯苓中落新妇苷质量分数为2.10%～5.92%。药效学实验结果表明,断面白色土茯苓抗炎抑制率为32%,断面红色土茯苓抗炎抑制率仅为21%。结论:红、白2种断面土茯苓中落新妇苷的含量及抗炎作用均存在较大差异,土茯苓中落新妇苷的含量高低与其抗炎作用的强弱不呈正相关。Objective： Astilbin in 28 Smilax glabra （red and white cross-section） from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment（penetration type）. Method： The analysis was performed on a Hypersil ODS2 column （4. 6 mm× 250 mm, 5 μm） with the mobile phase of acetonitrile and 0. 1% acetic acid aqueous （21： 79） at a flow rate of 1.0 mL · min^-1. The detection wavelength was 291 nm, and the column temperature was 25℃. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment. Result： Linear correlation was good in the range of 0. 003 379-4, 004μg,and the average recoveries were 100. 1% ,101.9% ,99. 3% ,respectively. The content of astilbin in white and red transverse section were 0. 19% -2. 46% and 2. 10% -5.92% ,respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%, respectively. Conclusion： Astilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra, the content of astilbin is not positively related with anti-inflammatory effect.

关 键 词：土茯苓(断面红色、白色) 落新妇苷 HPLC 抗炎试验 

分 类 号：R284[医药卫生—中药学]