川芎吸收入脑成分的UPLC-Q-TOF-MS分析  

Analysis of chemical constituents of Chuanxiong Rhizoma absorbed into rat brain tissues by UPLC-Q-TOF-MS

作　　者：左爱华[1] 程孟春[1] 王莉[1] 肖红斌[1] 

机构地区：[1]中国科学院大连化学物理研究所分离分析重点实验室,辽宁大连116023

出　　处：《中国中药杂志》2012年第23期3647-3650,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973873)

摘　　要：目的:对大鼠灌胃川芎提取物后的吸收入脑成分进行分析鉴定。方法:采用UPLC-Q-TOF-MS对给药脑组织和空白脑组织进行分析,分析总离子流图寻找差异峰,然后根据保留时间、精确分子量、一级和二级质谱数据等鉴定差异峰。结果:川芎提取物给药后,发现3个化合物能透过血脑屏障进入脑组织,经鉴定为洋川芎内酯I,A和藁本内酯。结论:本研究将有助于川芎药效物质基础的阐明。Objective： To analyze and identify the chemical constituents in rat brain tissues after oral administration of Chuanxlong Rhizoma extracts. Method： The dosed and blank rat brain tissues were analyzed by UPLC-Q-TOF-MS. Different peaks were observed in total ion chromatograms and then identified according to their retention time, accurate mass weight, MS and MS/MS data. Result： After oral administration of Chuanxiong Rhizoma extracts, 3 compounds were absorbed into rat brain tissues through BBB. They were identified as senkyunolide I, senkyunolide A and ligustilide. Conclusion： The study is helpful for interpreting effective sub- stance of Ligusticum chuanxiong.

关 键 词：川芎 脑组织 化学成分 鉴定 

分 类 号：R285[医药卫生—中药学]