儿童链球菌感染分离株emm分型及超抗原基因谱的变迁  

Changes of emm Types and Superantigens of Streptococcus Pyogenes Isolated from Children

作　　者：赵成松[1] 马耀玲[1] 林弘睿[1] 冯丽娟[1] 梁云梅[1] 俞桑洁[1] 姚开虎[1] 高微[1] 袁林[1] 李艳[1] 杨永弘[1] 沈叙庄[1] 

机构地区：[1]首都医科大学附属北京儿童医院儿内科,北京100045

出　　处：《实用儿科临床杂志》2012年第22期1730-1732,1744,共4页Journal of Applied Clinical Pediatrics

基　　金：国家863计划(2006AA02Z417)

摘　　要：目的通过对1993-1994年和2005-2006年359株儿童A族溶血性链球菌(GAS)分离株进行emm分型及8种超抗原基因检测,探讨不同时期儿童GAS感染分离株emm分型及超抗原基因的变化。方法采用PCR法对2个时期359株儿童GAS分离株进行emm分型及8种超抗原基因扩增。结果 emm1和emm12在不同时期均为主要流行型,其他型别不断变化。与1993-1994年比较,2005-2006年GAS菌株emm型较集中,emm1和emm12显著增多(Pa<0.01)。携带6种或6种以上超抗原基因的GAS分离株从46.53%(1993-1994年)增加到78.39%(2005-2006年),ssa,speH和speJ基因的携带率明显增加(P<0.05),而speA基因的携带率显著下降(P<0.05)。超抗原emm基因谱与emm分型之间有密切关系,相同的emm型在不同的时期携带不同的超抗原基因,emm型的分布和超抗原基因谱与GAS感染性疾病之间无显著性联系。结论儿童链球菌感染分离株emm分型及超抗原基因谱随年代变迁。此数据为制备符合我国GAS感染的疫苗提供依据。Objective To explore the variation of emm types and superantigens in different periods（ 1993 - 1994,2005 - 2006） in chil- dren through testing the emm types and 8 superantigen genes of 359 group A streptococal isolates （GSA）. Method Superantigen genes and emm genes were amplified by means of PCR for 359 GAS isolates collected from Chinese pediatric patients in 2 periods （ 1993 - 1994,2005 - 2006）. Results Types of emml and emml2 were consistently the most prevalent during the two periods, while others varied in frequency. Emm types were more concentrated in emml and emml2 （Pa 〈 0.01 ） in 2005 -2006 than those in 1993 - 1994. GAS isolates carr-ying six or more superantigen genes increased from 46.53% （ 1993 - 1994） to 78.39% （2005 - 2006） ; ssa, spell and speJ genes increased （ P 〈 0.05 ） but speA significantly declined （ Pa 〈 0.05 ）. Superantigen gene profiles were closely associated with the emm type, but strains of the same emm type sometimes carried different superantigen genes in 2 periods. No significant difference in emm - type distribution and superantigen gene profile was noted between isolates from different diseases. Conclusions Change of emm types and superantigen of streptococcus pyo- genes isolates is obviously in different periods in children. These data may contribute towards the development of a GAS vaccine in China.

关 键 词：emm分型 流行病学 化脓性链球菌 超抗原 

分 类 号：R440[医药卫生—诊断学]