锝气体肺通气显像在家兔急性肺栓塞模型制备中的作用  

The role of Technegas pulmonary ventilation imaging in establishing rabbit models of acute pulmonary embolism

作　　者：蒋仁容[1] 汤小惠[1] 

机构地区：[1]第三军医大学新桥医院核医学科,重庆400037

出　　处：《重庆医学》2012年第33期3512-3514,F0003,F0004,共5页Chongqing medicine

基　　金：重庆市自然科学基金资助项目(CSCT2009BB4146)

摘　　要：目的探讨颈静脉插管、聚酯海绵栓子制备实验家兔急性肺栓塞模型的可行性;研究实验家兔锝气体(Technegas)肺通气显像的技术方法,初步评价肺通气显像的成像质量。方法 20只实验组家兔抽签法随机分为2h组、24h组、3d组和7d组4个实验组,每组5只,另设2只作为对照组。每只实验组家兔经颈静脉插管注入4个聚酯海绵栓子,术后按设定时间分组行Technegas肺通气显像,显像结束后行病理解剖;对照组插管注入生理盐水,于第1天和第3天对照观察。结果 24h组1只家兔术中死亡,19只建模成功,成功率95%。实验组及对照组家兔均顺利完成Technegas肺通气显像,肺轮廓显像均清楚。实验组家兔处死后共解剖肺动脉285支,发现栓子74个,栓塞肺动脉61支。结论颈静脉插管、聚酯海绵栓子制备实验家兔急性肺栓塞模型,其操作简便、重复性好、成功率高。实验家兔Technegas肺通气显像经改良后简单易行,成像质量满意。Objective To explore the feasibility of creating rabbit acute pulmonary embolism model by jugular vena catheter using polyester sponge for emboli.To study the methods of Technegas pulmonary ventilation imaging and preliminary evaluate the image quality.Methods Twenty experimental rabbits were randomly divided into 2 groups,that is,the experimental group（n=20）,and the control group（n=2）.The rabbits of experimental group were further equally randomized into 4 subgroups,2-hour group,24-hour group,3-day group and 7-day group.The animal model was prepared by jugular vena catheter using polyester sponges as emboli.Pulmonary ventilation imaging was performed after the operation,then the rabbits were killed at the corresponding time points and the pulmonary tissue were pathological inspected to search and record the numbers and location of emboli.The control group rabbits were injected physiological saline solution via catheter after operation,and killed at 1 day and 3 days respectively.Results One rabbitdied during the operation,and the left 19 of 20 rabbits were made as acute pulmonary embolism model successfully.Totally 285 pulmonary arteries were anatomized after Technegas imaging.74 emboli were found and 61 pulmonary embolisms were made.All of the 19 rabbits were undergoing pulmonary ventilation imaging with different time and got clear images respectively.Conclusion The method of creating rabbits acute pulmonary embolism by jugular vena catheter using polyester sponges as emboli is simple and with high successful rate.The method for Technegas pulmonary ventilation imaging used in the research is simple and the image quality is satisfactory.

关 键 词：肺栓塞 动物模型 肺通气显像 

分 类 号：R563.5[医药卫生—呼吸系统]