幼年猪半月板体外器官培养模型的建立  

In vitro meniscal culture of juvenile porcine

作　　者：戴祝[1] 陈志伟[1] 杨乐忠[1] 刘春磊[1] 

机构地区：[1]南华大学附属第一医院骨科,湖南衡阳421001

出　　处：《中国骨伤》2012年第11期937-940,共4页China Journal of Orthopaedics and Traumatology

基　　金：2010年湖南省自然科学基金(编号:10JJ3094)~~

摘　　要：目的:观察幼年猪半月板在体外器官培养条件下的组织变化特点,为在体外器官培养下进行半月板研究提供实验依据。方法:1月龄幼年猪8头,取双膝内、外侧半月板,从每个半月板体部横行切取宽约8mm标本1份,共32份标本,进行体外器官培养。分别于培养0、2、4、6周后随机取出8个标本行组织学检查,HE染色观察半月板内侧1/3区域,计数每个视野的细胞数,比较各时间点间的差异;番红O染色观察半月板内侧1/3区域,记录染色程度,半定量评分,比较各时间点间的差异。结果:培养0、2、4、6周后,各时间点间每高倍视野的细胞数差异有统计学意义(P<0.05),两两比较差异均有统计学意义(P<0.05)。培养0、2、4、6周后,各时间点间番红O染色半定量评分差异有统计学意义(P<0.05),两两比较差异均有统计学意义(P<0.05)。结论:随体外器官培养时间延长,幼年半月板软骨细胞密度及活性逐渐下降。体外器官培养模型适合半月板的短期研究。Objective：To observe the histological changes of juvenile porcine menisci in an in vitro organ culture system. It was hoped that the experiment can provide evidence for the study of menisci in an in vitro organ culture model. Methods： Eight juvenile swines of one month old were involves in the study. Thirty two meniscal specimens with 8 mm in width were obtained from each meniscus in the two posterior extremities. The specimens were incubated in an in vitro organ culture model. At 0,2,4 and 6 weeks after culture,8 specimens were observed. Hematoxylin eosin staining：the cell density of the inner 1 / 3 zone was measured. Safranin-O staining：the degree of staining in the inner zone was measured and semi quantitative scores were obtained. Results：At 0,2,4 and 6 weeks,the cell density were （285.3±12.0） / HPF,（182.8±11.2） / HPF,（129.7±9.9） / HPF, （92.3±9.3） / HPF respectively. Statistically significant differences were found between different time points （P〈0.05）,Paired comparison showed statistical differences （P〈0.05）. At 0,2,4 and 6 weeks,the semi quantitative scores of Safranin-O staining were 2.0±0.0,1.5±0.5,1.0±0.0,0.5±0.5,statistically differences were found between different time points （P〈0.05）. Paired comparison showed statistical differences （P〈0.05）. Conclusion：In the juvenile meniscus in vitro organ culture model,the cell density and viability diminished along with the cultural time. In vitro organ culture model is suitable for the study of menisci in short term.

关 键 词：半月板 胫骨 模型 动物 软骨细胞 器官培养技术 猪 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]