毛细管区带电泳-多波长法测定甘草中6种有效成分的含量  

Separation and determination of six active components by HPCE and multi-wavelength method in licorice

作　　者：尚晓娜[1] 宋平顺 杨锡 何禄仁 赵建邦[1] 丁永辉 鲁静[4] 林瑞超[4] 

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]甘肃省食品药品检验所甘肃省中药品质与安全评价工程技术研究中心,甘肃兰州730000 [3]甘肃省食品药品监督管理局,甘肃兰州730000 [4]中国食品药品检定研究院,北京100010

出　　处：《中国医院药学杂志》2012年第23期1866-1869,共4页Chinese Journal of Hospital Pharmacy

基　　金："十一五"国家科技支撑计划项目(编号:2009ZX09308-001);甘肃省科技重大专项计划项目(编号:1002KFDA048)

摘　　要：目的:采用高效毛细管电泳分离及多波长采集法测定甘草药材中6种有效成分的含量。方法:以70 mmol.L-1的硼酸钠缓冲液(pH9.2)为电泳介质,未涂层弹性石英毛细管(75μm×64.5 cm,有效分离长度56 cm)为分离通道,压力进样(150Mpa.S),20 kV恒压电泳(20℃)分离,不同的波长段检测。结果:整个分析在15 min内完成,甘草次酸、甘草苷、甘草查尔酮A、甘草素、甘草酸及异甘草素均得到良好的分离且在各自浓度范围内均具有良好的线性关系。结论:本实验建立的HPCE同时测定黄酮类、三萜类成分的含量方法准确、简便,可作为甘草质量控制的方法之一。OBJECTIVE To develop a method by high performance capillary electrophoresis （HPCE） for separating and de termining the six active contents in licorice from different areas. METHODS The dimensions of the capillary tube used in HPCE analysis were 64. 5 cm× 75μm and the effective length was 56 cm The temperature of the capillary tube was maintained at 20 ℃. The buffer solution contained 70 mmol. L- 1 sodium borate at a pH of 9. 2. All CE runs were made at an applied voltage of 20 kV, and with a multi-wavelength method detection. RESULTS Glycyrrhetinic acid, glycyrrhizin, licorice chalcone A, liquiritigenin, glycyrrhizin acid and isoliquiritigenin were successfully separated in 15 min , and all had good linear relationship in their respective range of concentration. CONCLUSION The methods established for simultaneous determinating of fla vonoids and triterpenoid content by HPCE was accurate and simple, which could be one of the methods of quality control of Licorice.

关 键 词：高效毛细管电泳法 多波长法 甘草 黄酮类 三萜类 

分 类 号：R927.2[医药卫生—药学]