非诺贝特对慢性心力衰竭大鼠心肌能量代谢和心室重构的影响  

Effect of fenofibrate on myocardial metabolism and ventricular remodeling in rats with chronic heart failure

作　　者：邬江涛[1] 何燕[1] 吴龙飞[1] 钟国强[1] 李醒三[1] 王炎 陈卓宏[1] 闭奇[1] 

机构地区：[1]广西医科大学第一附属医院老年心内科,南宁530021

出　　处：《中华老年心脑血管病杂志》2012年第12期1302-1307,共6页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广西壮族自治区科技厅青年基金项目(0728064)

摘　　要：目的研究非诺贝特对慢性心力衰竭(CHF)大鼠心肌能量代谢和心室重构的影响。方法健康雄性Wistar大鼠随机选为假手术组18只;采用腹主动脉缩窄术制备CHF、并成功存活的38只再随机分为对照组20只、非诺贝特组18只[非诺贝特150 mg/(kg·d)],干预10周。计算左心室心肌重构指数、胶原容积分数(CVF)、心肌线粒体损伤程度分级用Flameng评分,免疫印迹法测过氧化物酶体增殖物激活受体α(PPARα)、中链酰基辅酶A脱氢酶(MCAD)、肌型肉碱棕榈酰转移酶1(MCPT 1)蛋白表达,RT-PCR测PPARα、MCAD和MCPT-1mRNA表达。结果与假手术组比较,对照组及非诺贝特组左心室心肌重构指数、CVF和Flameng评分均升高(P<0.05);非诺贝特组左心室心肌重构指数高于对照组、CVF和Flameng评分低于对照组(P<0.05);与假手术组比较,对照组、非诺贝特组心肌PPARα、MCPT-1、MCAD蛋白和基因表达均下调(P<0.05);非诺贝特组表达较对照组上调(P<0.05)。结论非诺贝特通过增强脂肪酸氧化,减轻线粒体损伤,改善心室重构,减轻心肌纤维化。Objective To study the eftect ot ienolibrate on myocardial metabolism and ventricular remodeling in rats with chronic heart failure（CHF）. Methods Eighteen healthy male Wistar rats served as a sham operation group in this study. A CHF model was established by constricting the abdominal artery. Thirty-eight survived rats were rondomly divided into control group（n：20）and fenofibrate group[n= 18, fenofibrate150 rag/（kg . d）, for 10 weeks]. Left ventricular myocardial remolding index and CVF were culculated. Myocardial mitochondrial injury was scored according to the Flameng scale. Expressions of PPAR,MCAD,MCPT-1 protein and mRNA were detected by Western blot and RT-PCR,respectively. Results The left ventricular myocardial remolding index, CVF and myocardial mitoehondrial injury score were significantly higher in control and fenofibrate groups than in sham operation group（P〈0.05）. The left ventricular myocardial remolding index was significantly higher whereas the CVF and myocardial mitochontia injury score were sig- nificantly lower in fenofibrate group than in control group （P（0.05）. The expression levels of PPARa,MCPT-1 and MCAD protein were significantly lower in control and fenofibrate groups than in sham operation group and significantly higher in fenofibrate group than in sham operation group（P（0.05）. Conclusion Fenofibrate can alleviate mitochondrial injury,improve left ventricular remodeling and reduce myocardiac fibrosis by oxidating fatty acids.

关 键 词：心力衰竭 普鲁脂芬 心室重构 线粒体 心脏 血流动力学 

分 类 号：R541.6[医药卫生—心血管疾病]