粘液繁殖体植物抱茎独行菜TTG1基因转化拟南芥的表型研究  

Phenotype of Transgenic Arabidopsis thaliana with TTG1 Gene from Myxospermy Plant Species Lepidium perfoliatum

作　　者：贺转转[1] 王文艳[1] 黄代红[1] 徐栋生[1] 兰海燕[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2012年第11期2048-2053,共6页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(30860020);国家教育部留学回国人员启动基金项目(2006-2009)

摘　　要：[目的]将过量表达典型粘液繁殖体植物抱茎独行菜TTG1基因(LpTTG1)的转基因拟南芥株系表型与野生型和突变体进行比较,为深入研究LpTTG1基因的功能提供参考依据。[方法]在体视显微镜下,通过对拟南芥野生型、ttg1突变体及LpTTG1基因过表达转基因株系的植株表型,幼苗叶表皮毛、下胚轴和根毛以及种子形态进行观察比较。[结果]拟南芥ttg1突变体与野生型和LpTTG1过表达转基因型的幼苗在下胚轴的颜色、根毛着生方式、真叶表皮毛等方面有显著差异,但后两者表型间无显著差异。[结论]缺失TTG1基因可导致拟南芥幼苗形态发生显著变化,但此基因过量表达不一定对拟南芥形态产生影响。[ Objective ] To study the phenotype change in transgenic Arabidopsis with LpTTG1 gene （from Lepidium perfoliatum, a typical myxospermy species）overexpression, and in hope of being able to provide evidence for exploring the biological functions of LpTTG1. [ Method] With stereomicroscope, the plant phenotype, trichome of young seedling leaf, hypocotyl color, root hairs and seed morphology were observed in wild - type, ttgl mutant and overexpression LpTTG1 gene Arabidopsis. [ Result] The color of hypocotyl, the way of root hair growth and the leaf trichome presented obvious difference between ttgl mutant and wild - type or LpTTG1 overexpression Arabidopsis, however, no significant difference was observed between wild - type and LpTTG1 overexpression Arabidopsis. [ Conclusion ] Loss of TTG1 gene can cause apparent changes in morphology of Arabidopsis seedling, while overexpression of this gene is not likely to affect the morphology of Arabidopsis.

关 键 词：粘液繁殖体 抱茎独行菜 TTG1基因 拟南芥 表型 

分 类 号：Q948[生物学—植物学]