黄花美冠兰花芽分化过程中假鳞茎内源激素含量变化的研究  

Study on Changes of Endogenous Hormone Contents in Pseudobulbs During the Floral Bud Differentiation of Eulophia flava

作　　者：杨光穗[1] 黄素荣[1] 王存[1] 黄少华[1] 张欢[2] 张志群[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]海南大学园艺园林学院,海南海口570228

出　　处：《北方园艺》2012年第23期75-78,共4页Northern Horticulture

基　　金：国家公益性农业行业科技资助项目(201203071);农业生物资源保护与利用资助项目(2130135)

摘　　要：采用酶联免疫吸附测定法(ELISA)测定了花芽分化过程中黄花美冠兰假鳞茎内源激素吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA)和玉米素核苷(ZRs)含量的动态变化。结果表明:假鳞茎内IAA和ABA的含量明显高于GA和ZRs的含量,花芽分化过程中,假鳞茎内IAA和GA的含量下降,ABA和ZRs的含量上升,ZRs/IAA、ZRs/GA、ABA/IAA、ABA/GA的比值均呈上升趋势,ABA/IAA、ABA/GA的比值升幅高于ZRs/IAA、ZRs/GA的比值。During the floral bud differentiation of Eulophia flava, four kinds of endogenous hormones IAA, ABA,GA, ZRs contents in pseudobulb were measured with the enzyme-linked immunosorbent assay （ELISA）. The results showed that in the process of the floral bud differentiation, ABA content and ZRs cpmtent in the pseudobulb increased,while IAA content and GA content decreased. The ratio of ZRs to IAA,ZRs to GA, ABA to IAA,ABA to GA presented uptrend, besides,ABA to IAA and ABA to GA leaded a higher ratio than ZRs to IAA and ZRs to GA.

关 键 词：黄花美冠兰 花芽分化 内源激素 

分 类 号：S682.31[农业科学—观赏园艺]