不同方法提取野生荔枝基因组DNA效果的比较  

Comparison on Effects of Different Methods on Extract Genomic DNA of Wild Litchi Leaves

作　　者：刘锴栋[1] 袁长春[1] 陈燕[1] 莫秋梅[1] 黎海利[1] 

机构地区：[1]湛江师范学院生命科学与技术学院,广东湛江524048

出　　处：《北方园艺》2012年第23期105-109,共5页Northern Horticulture

基　　金：广东高校优秀青年创新人才培养计划资助项目(LYM11087);广东省科技计划资助项目(2008B020300001);湛江市科技攻关计划资助项目(2011C3106019;2012C3102019);湛江师范学院青年科研基金资助项目(QL0911)

摘　　要：以广东、广西地区的3个野生龙眼的幼嫩叶片为试材,采用SDS法、常规CTAB法和改良2×CTAB法提取荔枝叶片基因组DNA,比较研究从富含多酚、多糖和色素等次生代谢物质的野生荔枝叶片中获得高质量DNA的提取方法。结果表明:采用改良的2×CTAB法提取的荔枝叶片基因组DNA纯度最高、质量最佳,可直接用于荔枝叶绿体DNAtrnL-F基因的PCR扩增分析,且扩增后条带清晰。表明改良的2×CTAB方法获得的DNA纯度和产率较高,符合进行野生分子标记的实验要求。Experiment was conducted with three different leaves of wild litchi from Guangdong and Guangxi province. The genomic DNA extraction of three methods namely SDS, CTAB and modified 2 ×CTAB were compared. The aim was to obtain the high quality DNA extracted method from leaves of wild litchi which contained plenty of secondary substances such as polysaccharide, polyphenols, pigment and protein. The results showed that the modified 2 ×CTAB method could extract the genomic DNA from wild litchi leaves of the highest purity and best ,quality. It also could apply on the PCR amplificative analysis on the cpDNA trnL-F gene of wild litchi leaves with distinct strips after amplification. Therefore, the modified 2 ×CTAB method was the better method to extract the geriomic DNA from wild litchi leaves.

关 键 词：野生荔枝 基因组DNA SDS法 CTAB法 

分 类 号：S667.1[农业科学—果树学]