PTA-1^(-/-)/ApoE^(-/-)双基因敲除小鼠的繁育及基因型鉴定  

Breeding and Genotyping of PTA-1^(-/-)/ApoE^(-/-)Double-gene Knockout Mice

作　　者：董子龙[1] 庄然[2] 张宇丝[2] 金伯泉[2] 张圆[3] 

机构地区：[1]第四军医大学西京医院皮肤科,西安710032 [2]第四军医大学免疫学教研室,西安710032 [3]第四军医大学生理学教研室,西安710032

出　　处：《实验动物科学》2012年第5期6-8,共3页Laboratory Animal Science

摘　　要：目的建立(PTA-1-/-/ApoE-/-)双基因敲除小鼠(double-gene knockout,DKO)模型,探讨该小鼠的繁育及鉴定方法,为进一步利用该小鼠研究相关疾病奠定基础。方法将引进的PTA-1-/-及ApoE-/-基因敲除小鼠通过杂交和互交的方法进行繁殖,以得到DKO小鼠。结果经过PCR基因鉴定的方法证实PTA-1-/-/ApoE-/-双基因敲除小鼠繁育成功。结论正确的饲养繁殖及鉴定方法是获得该DKO纯合子小鼠的有效途径。Objective To breed and identify the PTA-1 -/-/ApoE -/- double-gene knockout （DKO） mice and to establish an animal model to further study the role of PTA-1 molecule in diseases. Method PTA-1 gene knockout mice were paired with the ApoE gene knockout mice in different ways. Genomie DNA were isolated from the tails and analyzed by PCR. Result Genotyping analysis identified that we established PTA-1 -/-/ApoE-/- DKO mice successfully. Conclusion It is feasible to breed PTA-1 -/-/ApoE-/- DKO mice with the PTA-1 and ApoE gene knockout mice. PCR can be used to identify the genotype of the DKO mice precisely

关 键 词：PTA-1 APOE 双基因敲除 小鼠 

分 类 号：Q95-33[生物学—动物学]