不同保存方法对RT-PCR检测蜜蜂病毒的影响  

Impact of Different Preservation Condition on the Detection of Bee Virus by RT-PCR

作　　者：马啸天[1] 吴孟洁[1] 毕峻龙[2] 赵文正[1] 和绍禹[1] 

机构地区：[1]云南农业大学东方蜜蜂研究所,云南昆明650201 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《蜜蜂杂志》2012年第12期1-3,共3页Journal of Bee

基　　金：国家农业产业技术体系(蜜蜂)(CARS-45-ksj14)

摘　　要：本研究从已知感染蜜蜂黑王台病毒(Blackqueen cell virus;BQCV)和蜜蜂畸翅病毒(Deformed WingVirus,DWV)的东方蜜蜂蜂群中采集工蜂个体,将其纵向剖成3份,一份存于-79℃,一份加入1.2 mL无水乙醇保存,第三份加入1.2 mL TRIzol保存。对3组样本每隔3 d同时进行RNA提取,并使用RT-PCR方法检测蜜蜂黑王台病毒和蜜蜂畸翅病毒。结果表明在TRIzol中保存的样品和-79℃冰冻保存的样本所提取RNA相对完整,病毒检测结果一致;在无水乙醇中保存的样品RNA质量相对较差,病毒检测阳性率低于前两者。该研究为蜜蜂病毒检测的采样保存方法提供一定的理论依据。For the present study, samples of bee worker were collected from the Apis terra colonies infected with black queen cell virus （BQCV） and deformed wing virus （DWV）. Each sample was sliced into three pieces and one of which was stored at -79℃, the other two were kept in 1.2mL 100% ethanol and 1.2mLTRIzol respectively. Aiming at these three different storage methods, RNA extractions and detections of BQCV and DWV using RT-PCR were carried out with each piece at an interval of three days. Analysis of the results indicating that the integrity of RNA extracted from the pieces stored at -79℃ and in TRIzol was rela- tively in good condition while it was not so good for the piece kept in 100% ethanol. Detections of viruses exhibit-ed the same trend.

关 键 词：蜜蜂病毒 保存方法 RT-PCR 

分 类 号：S895[农业科学—特种经济动物饲养]