Overexpression of OsDPR, a novel rice gene highly expressed under iron deficiency, suppresses plant growth  

作　　者：Naren ZHANG Peng MA DengKe WANG Yi LI Shuang YIN LiPing 

机构地区：[1]College of Life Science, Capital Normal University, Beijing 100048, China

出　　处：《Science China(Life Sciences)》2012年第12期1082-1091,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30170552);the Academic and Technical Development Key Project Foundation of Beijing Education Community (Grant No. KZ200710028013)

摘　　要：Preliminary microarray analysis of cDNA from rice roots revealed an up-regulated transcript that was highly expressed in a five-day iron deficiency treatment. The entire sequence of this gene was determined by bioinformatics analysis. There were no proteins with significant levels of similarity detected in public databases. This novel gene with unknown biological function was designated as OsDPR (dwarf phenotype-related gene). We constructed a stable plant expression vector pCAM- BIA1302-OsDPR::GFP and produced transgenic tobacco plants. The phenotypes suggested that OsDPR restrained the growth of transformed plants. To understand the mechanisms of this suppression effect, cell size and number were compared between transformants and wild-type plants. The cell proliferation rate was lower in OsDPR transgenic BY-2 cells than in wild-type cells, but OsDPR expression did not affect cell size. Moreover, the cell division-related gene CyclinD2.1, which is involved in plant growth, was down-regulated in transgenic tobacco plants. These findings suggested that the novel iron-regulated gene OsDPR is responsible for the nanism phenotype of transgenic seedlings because of the inhibition of plant cell proliferation.Preliminary microarray analysis of cDNA from rice roots revealed an up-regulated transcript that was highly expressed in a five-day iron deficiency treatment. The entire sequence of this gene was determined by bioinformatics analysis. There were no proteins with significant levels of similarity detected in public databases. This novel gene with unknown biological function was designated as OsDPR （dwarf phenotype-related gene）. We constructed a stable plant expression vector pCAM- B IA 1302-OsDPR：：GFP and produced transgenic tobacco plants. The phenotypes suggested that OsDPR restrained the growth of transformed plants. To understand the mechanisms of this suppression effect, cell size and number were compared between transformants and wild-type plants. The cell proliferation rate was lower in OsDPR transgenic BY-2 cells than in wild-type cells, but OsDPR expression did not affect cell size. Moreover, the cell division-related gene CyclinD2.1, which is involved in plant growth, was down-regulated in transgenic tobacco plants. These findings suggested that the novel iron-regulated gene OsDPR is responsible for the nanism phenotype of transgenic seedlings because of the inhibition of plant cell proliferation.

关 键 词：iron deficiency restrained growth cell division CyclinD2.1 

分 类 号：S858.28[农业科学—临床兽医学] Q945.3[农业科学—兽医学]