红景天苷贴心肌缺血／再灌注损伤大鼠内皮素-1的影响  

The effect of Salidroside on endothelin-1 in rats with acute myocardial ischemia/reperfusion injury

作　　者：董化江[1,3] 单娜娜[2] 罗悦晨[3] 徐健[1] 赵明亮[3] 徐鹏宵 杨德慧[1] 李伯森[1] 单云官[1] 臧照辉[4] 

机构地区：[1]武警后勤学院解剖教研室,民政部101研究所遗体防腐整容重点实验室,天津300162 [2]武警后勤学院教育技术中心,天津300162 [3]武警后勤学院附属医院,天津300162 [4]武警后勤学院研究生处,天津300162

出　　处：《中国中西医结合急救杂志》2012年第6期333-335,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：“十一五”国家科技支撑计划（2007BAK38805）

摘　　要：目的探讨红景天苷（Sa）对大鼠心肌缺血／再灌注（I／R）损伤后内皮素-1（ET-1）的影响及其保护机制。方法将60只Wistar大鼠按随机数字表法均分为假手术组、缺血组、I／R1h组、I／R2h组、Sa预处理1h组和Sa预处理2h组。采用结扎冠状动脉（冠脉）前降支30min后恢复血流灌注复制I／R损伤大鼠模型。Sa组于术前20min舌下静脉给予Sa 25mg／kg预处理。相应时间点观察假手术组、缺血组、I／R组心电图ST段抬高幅度；采用酶联免疫吸附试验（ELISA）测定各组血清肌酸激酶（CK）、乳酸脱氢酶（LDH）的活性，放射免疫试验测定血清内皮素-1（ET-1）水平，逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹法（Western blotting）检测各组心肌组织ET-1 mRNA及蛋白表达。结果缺血组和I／R组ST段抬高幅度及血清CK、LDH均较假手术组显著升高；I／R组ST段抬高幅度较缺血组显著降低，CK、LDH则较缺血组显著升高。与假手术组比较，缺血组、I／R1h组、I／R2h组、Sa预处理1h组、Sa预处理2h组血清ET-1（μg/L）水平均明显升高（66．49±8．61、104．60±6．64、108．82±6．22、71．30±12．22、69．60±7．40比24．58±3．41，均P〈0．05）；心肌ET-1蛋白表达（灰度值）明显升高（0．51±0．07、0．76±0．08、0．77±0．06、0．53±0．09、0．58±0．04比0．34±0．10，均P〈0．05）；I／R1h组、I／R2h组、Sa预处理1h组、Sa预处理2h组ET-1mRNA表达[吸光度似）值]均明显升高（0．73±0．04、0．78±0．02、0．51±0．06、0．55±0．03比0．31±0．07，均P〈0．05），而缺血组则差异无统计学意义（P〉0．05）。与缺血组比较，I／R1h组、I／R2h组血清ET-1水平、心肌组织ET-1 mRNA和蛋白表达均明显升高（均P〈0．05）；Sa预处理1h组、Sa预处理2h组血清ET水平及心肌组织ET-1 mRNA和蛋白表达均较I／R1h组、I／R2h组明显下降（均P〈0．05）。结论ETObjective To investigate the effect of Salidroside （Sa） on changes of endothelin-1 （ET-1） in rats after myocardial ischemia/reperfusion （I/R） injury and its protective mechanism. Methods Sixty Wistar rats were grouped randomly into sham group （S）, ischemic 30 minutes group （I）, ischemic 30 minutes ＋ reperfusion 1-hour group （I/R 1 h）, ischemic 30 minutes ＋ reperfusion 2-hour group （I/R 2 h）, Sa ＋ ischemic 30 minutes ＋ reperfusion 1-hour group （Sa＋I/R 1 h） and Sa ＋ ischemic 30 minutes ＋ reperfusion 2-hour group （Sa＋I/R 2 h）. Myocardial I/R injury model was reproduced by 30-minute ligation of the left anterior descending branch, followed by reperfusion after ischemia. In Sa ＋ I/R groups, 25 mg/kg Sa was given in sublingual vein 20 minutes before surgery. The ST-segments amplitude of electrocardiogram （ECG）in S, I and various I/R groups was observed at different time points. The activities of creatine kinase （CK）and lactate dehydrogenase （LDH） were examined by enzyme-linked immunosorbent assay （ELISA）. The changes of ET-1 were evaluated by radioimmunoassay, cardiac tissue ET-1 mRNA was determined by reverse transcriptase-polymerase chain reaction （RT-PCR）and its protein was measured by Western blotting. Results The ST-segments amplitude of ECG, CK and LDH in serum in I and I/R groups were increased significantly compared with those in S group. The magnitude of ST-segment elevation was lower significantly in I/R groups than that in I group, while CK and LDH in serum in I/R groups were obviously higher than those in I group. Compared with S group, the serum levels of ET-1 （μg/L） were increased in groups of I, I/R 1 h, I/R 2 h, Sa ＋ I/R 1 h and Sa ＋ I/R 2 h （66.49±8.61,104.60±6.64,108.82±6.22,71.30±12.22,69.60±7.40 vs. 24.58±3.41,all P〈0.05）, the protein expression of ET-1 （gray scale）in myocardial tissue was increased significantly （0.51±0.07, 0.76±0.08, 0.77±0.06,0.53±0.09,0.58±0.04 vs. 0.34±0.10, all P〈0.05�

关 键 词：红景天苷 缺血 再灌注损伤 心肌 内皮素-1 大鼠 

分 类 号：R285.6[医药卫生—中药学] R256.2[医药卫生—中医学]