血必净注射液对硫化氢急性中毒大鼠肺组织细胞因子及核转录因子-κB的影响  

Effect of Xuebijing injection on cytokines and nuclear factor-K B in lung tissue of acute hydrogen sulfideintoxicated rats

作　　者：卢晗[1] 葛赟[2] 洪广亮[2] 姜小珍[2] 刘瑶[2] 邱俏檬[2] 卢中秋[2] 

机构地区：[1]温州医学院第二临床医学院,浙江温州325035 [2]温州医学院附属第一医院急诊科,浙江温州325000

出　　处：《中国中西医结合急救杂志》2012年第6期343-347,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省高校重点学科建设计划;浙江省医学创新学科建设计划（11-CX26）;浙江省中医药科学研究基金计划（2010ZA086）

摘　　要：目的探讨血必净注射液对硫化氢（H2S）急性中毒大鼠肺组织肿瘤坏死因子-α（TNF-α）、白细胞介素（IL-6、IL-10）及核转录因子-κB（NF—κB）的影响。方法将96只清洁级SD大鼠按随机数字表法分为正常对照组（n=8）、血必净对照组（n=8）、H：S染毒模型组（n=40）和血必净干预组（n=40）。采用酶联免疫吸附试验（ELISA）测定肺组织中TNF-α、IL-6、IL-107水平；用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blotting）检测肺组织NF-κB mRNA和蛋白表达；光镜下观察肺组织病理学改变。结果正常对照组和血必净对照组TNF-α、IL-6、IL-10水平及NF-κBmRNA和蛋白表达比较差异均无统计学意义（均P〉0．05）。与正常对照组比较，H2S染毒模型组在染毒后2、6、12、24、48h TNF-α（ng／L）、IL-6（ng／L）、IL-10（ng／L）水平及NF-κB mRNA和蛋白表达明显升高（均P〈0．01），分别于12、48、48、24、24h达峰值（TNF-α12h：400．79±13．74，IL-6 48h：242．69±8．96，IL-10 48h：353．73±10．75，NF-κB mRNA24h：0．568±0．007，NF-κB蛋白24h：0．520±0．007）；与H2S染毒模型组同期比较，血必净干预组TNF-α、IL-6水平及NF-κBmRNA和蛋白于2h开始即明显降低（TNF-α：322．46±10．16比377．89±10．87，IL-6：124．08±8．14比170．54±8．25，NF-κB mRNA：0．151±0．005比0．274±0．006，NF-κB蛋白：0．109±0．005比0．309±0．006，均P〈0．01），IL-10水平明显升高（361．94±11．04比321．80±13．86，P〈0．01）。光镜下可见24h时血必净干预组肺损伤程度明显减轻。结论血必净注射液干预能显著降低促炎因子TNF—α、IL-6水平及NF—κB mRNA和蛋白的表达，提高抗炎因子IL-10水平，减轻急性肺损伤。Objective To investigate the effect of Xuebijing injection on the levels of tumor necrosis factor- （TNF-α）, interleukin （IL-6, IL-10） and nuclear factor-κB （NF-κB） in the lung tissue of acute hydrogen sulfide （H2S）-intoxicated rats. Methods A total of 96 Sprague-Dawley （SD）rats of clean grade were divided randomly into four groups ： normal control group （n = 8）, Xuebijing control group （n = 8）, H2S-intoxieated model group （n = 40） and Xuebijing treatment group （n = 40）. After modeling, the levels of TNF-α, IL-6 and IL-10 in the lung tissue were measured by enzyme-linked immunosorbent assay （ELISA）methods, and the expression of NF-κB mRNA and protein in the lung tissue was detected by reverse transeription-polymerase chain reaction （RT-PCR） and Western blotting methods. Pathological changes of lung tissue were observed by light microscope. Results There was no significant difference between normal control group and Xuebijing control group in the levels of TNF-α, IL-6 and IL-10 and the expression of NF-κB mRNA and protein （all P〉0.05）. The TNF-α （ng/L）, IL-6 （ng/L）and IL-10 （ng/L） levels, and NF-κB mRNA and protein expression at 2,6, 12,24,48 hours in rats of H2S-intoxicated model group after modeling were markedly increased than those in normal control group （all P〈0.01） ; they reached the peak at 12, 48,48,24, 24 hours respectively （TNF-α 12 hours ： 400.79±13.74, IL-6 48 hours ： 242.69±8.96, IL-10 48 hours ： 353.73＋ 10.75, NF-κB mRNA 24 hours： 0.568±0.007, NF-KB protein 24 hours： 0.520±0.007）. In comparison with the same period of H2S-intoxicated model group, the TNF- α, IL-6 levels and NF-κB mRNA and protein expression at 2 hours in rats of Xuebijing treatment group decreased significantly （TNF-α ：322.46±10.16 vs. 377.89±10.87, IL-6 ： 124.08±8.14 vs. 170.54±8.25, NF-κB mRNA ： 0.151±0.005 vs. 0.274±0.006, NF-κB protein ： 0.109±0.005 vs. 0.309±0.006, all P〈0.01）, and the IL-10 levels at 2

关 键 词：硫化氢 血必净注射液 肺损伤 急性 核转录因子-ΚB 肿瘤坏死因子-α 白细胞介素 

分 类 号：R692[医药卫生—泌尿科学]