活性炭对鼎湖山紫背天葵组培苗生根的影响  

Effect of Activated Charcoal on Rooting in Tissue Culture Seedling of Begonia fimbristipula on Dinghushan Mountain

作　　者：陈雄伟[1] 邵玲[1] 梁廉[1] 潘镇涛[1] 

机构地区：[1]肇庆学院生命科学学院,广东肇庆526061

出　　处：《中药材》2012年第9期1369-1373,共5页Journal of Chinese Medicinal Materials

基　　金：广东省科技计划项目(2010B060200012)

摘　　要：目的:以带有球茎的不定芽为外植体,探讨不同植物生长物质与活性炭(AC)对鼎湖山紫背天葵组培苗生根和移栽的影响。方法:组织培养单因子试验法。结果:NAA 0.3 mg/L+IBA 0.2 mg/L的组合较好地诱导球茎芽丛生根,但根数仍较少,根细、短小,幼小的不定芽萌发数量较多。添加300 mg/L活性炭明显提高生根质量并且抑制细芽点的分化,根数多达15.5条,根长范围在2.0～5.1 cm,根系发达,并且组培苗植株较高、球茎和叶片质量佳,生长健壮。将生根的组培苗带球茎分散为丛芽的形式移栽到泥炭土-珍珠岩(3∶1)的混合基质中,移栽后成活率达到100%,植株出苗快,长势旺盛。结论:以MS+蔗糖30 g/L+NAA 0.3 mg/L+IBA 0.2 mg/L+活性炭300mg/L+卡拉胶7.0 g/L作为紫背天葵组培苗的生根体系,是离体快繁和保存本地特色植物的有效途径。Objective： To study the effect of different plant growth substance and activated charcoal on rooting in culture seedling of Begoniafimbristpula on Dinghushan mountain. Methods： Tissue culture single factor experiment method was used. Results ： NAA 0. 3 mg/L ＋ IBA 0. 2 mg/L preferably induction adventitious bud clump with corm to take rooting,but the number of adventitious root were less, short and small, callow shoot more germination. 300 mg/L activated carbon obviously increased radicate quality and inhibited fine buds point differentiation, root number up to 15. 5 institia, root length range was 2. 0 ~ 5. 1 cm, root system developed. Tissue culture seedlings were higher, corm and leaf were good quality, strong growth. Took root of seedling cultivation with bulb for bush in the form of scattered bud planted to peat soil： perlite （3：1 ） mixed in matrix, after the transplant survival rate reached 100%, plant form seedlings fast, grew exuberant. Conclusion： MS with sucrose 30 g/L ＋ NAA 0. 3 mg/L ＋ IBA 0. 2 mg/L ＋ activated carbon 300 mg/L ＋ carrag- eenan 7. 0 g/L as the tissue culture seedling of Begonia fimbristipula radicate system, is rapid propagation and preserve local unique plant in an effective way.

关 键 词：鼎湖山 紫背天葵 植物生长物质 活性炭 生根 

分 类 号：R282.2[医药卫生—中药学]