丹参HPCE-DAD指纹图谱的研究  

Study on HPCE-DAD Fingerprint of Salviae Miltiorrhizae Radix et Rhizoma

作　　者：宋建平[1] 许虎[2] 刘训红[2] 周逸芝[2] 陈龙[2] 傅兴圣[2] 李俊松[2] 蔡宝昌[2] 

机构地区：[1]盐城卫生职业技术学院,江苏盐城224006 [2]南京中医药大学江苏省中药炮制重点实验室,江苏南京210046

出　　处：《中药材》2012年第9期1414-1417,共4页Journal of Chinese Medicinal Materials

基　　金：江苏省中药炮制重点实验室开放式课题(ZYPZ007);江苏高校优势学科建设工程资助项目(ysxk-2010)

摘　　要：目的:建立丹参HPCE-DAD指纹图谱分析方法,评价丹参药材的质量。方法:用高效毛细管电泳技术对丹参药材进行分析,测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以10个共有峰为特征指纹信息的HPCE指纹图谱;发现少数丹参药材样品HPCE指纹图谱有一定差异。结论:方法准确可靠,重现性好,可作为丹参药材内在质量评价的依据。Objective ：To establish the analytical method for the fingerprint of Salviae Miltiorrhizae Radix et Rhizoma by HPCE- DAD and estimate its quality. Methods： Salviae Miltiorrhizae Radix et Rhizoma were analyzed and the chromatographic fingerprint were determined by HPCE-DAD. The data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software was used to compare the similarity of samples. Results： HPCE-DAD fingerprint of 10 main common peaks was established preliminarily. It was discovered that a small number of samples were different from the others. Conclusion： The method is reliable, accurate and can be used for the quality control of Salviae Mihiorrhizae Radix et Rhizoma.

关 键 词：丹参 高效毛细管电泳二极管阵列检测法 指纹图谱 

分 类 号：R282.5[医药卫生—中药学]