猪传染性胃肠炎病毒NC株的分离鉴定及生物学特性试验  

Isolation and identification of porcine transmissible gastroenteritis virus(TGEV) NC strain and analysis of biological characteristics

作　　者：钟望[1] 田野[1] 牛晓芸[1] 王艳丽[1] 张显浩 陈瑞爱[1,2] 贺东生[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省生物制品技术研究与应用企业重点实验室,广东肇庆526238

出　　处：《中国兽医杂志》2012年第11期13-17,共5页Chinese Journal of Veterinary Medicine

摘　　要：本试验成功分离了1株猪传染性胃肠炎病毒(TGEV),并对其进行了鉴定及生物学特性研究。取华南地区某猪场腹泻病猪肛拭子经无菌处理后接种PK-15细胞分离病毒,首代培养即产生典型的细胞病变。该毒株对鸡红细胞的血凝价为26,病毒效价(TCID50)为108.15/mL。经标准TGE阳性血清的血凝抑制和细胞中和试验鉴定该病毒为TGEV,血凝抑制滴度为28、中和效价为1∶381。通过RT-PCR检测及序列分析,确定其为TGEV,并将该毒株命名为TGEV NC株。TGEV NC株在没有超离纯化和4℃处理下仍有较高血凝特性,为国内首次报道。In this study, a TGE virus strain was successfully isolated and analyzed for its biological characteristics. Rectal swabs of piglets with clinical diarrhea symptom was collected, inoculated and cul- tured in PK-15 cell culture. Typical cytopathic effect appeared in cell cultures 3 days post inoculation. The RBC hemaggiutination titer of the isolated strain is 26. The virus titer is 108 TCIDso/mL. The strain was identified as TGEV by HI and SN test with TGE positive serum. The HI titer is 28 and the SN titer is 1 ： 381. The strain was named for TGEV NC strain after RT-PCR and sequence analysis. This study is the first report in China that TGEV has high hemagglutination without ultracentrifute purification and treatment in 4 ℃

关 键 词：猪传染性胃肠炎病毒 分离 血凝特性 鉴定分析 

分 类 号：S852.65[农业科学—基础兽医学]