探针的修饰与浓度杂交温度及时间对基因芯片杂交信号的影响  

Study on the impact of probe modification and concentration,hybridization temperature and time on gene chip hybridization signals

作　　者：刘玲玲[1] 唐桂芬[2] 李文刚[2] 饶宝 张永涛 程亚楼[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]郑州牧业工程高等专科学校,河南郑州450011 [3]牧原食品股份有限公司,河南南阳474360 [4]福建省建瓯市百世腾养猪发展有限公司,福建建瓯353100

出　　处：《中国兽医杂志》2012年第11期17-19,I0002,共4页Chinese Journal of Veterinary Medicine

基　　金：河南省科技厅科技攻关项目(092102110026)

摘　　要：本研究以猪链球菌2型的cps2J基因为试验材料,研究了两种探针在不同杂交条件下对基因芯片杂交信号的影响,试验结果表明,寡核苷酸探针的浓度在5～20μm/L之间对杂交信号的影响不大,寡核苷酸探针的氨基化端加上Poly(dT),42℃杂交1h可以获得较好的杂交信号。In the pagy, our study is focused on the influence of two probes on gene chip hybridization signals under different conditions of hybridization using Streptococcus suis II cps2J gene as an experimental material. Results showed that oligonucleotide probes whose concentration was between 5～20μm/L havelittle effect on hybridization signals;however better hybridization signals can be achieved by adding Poly （dT） to the amino-terminal end of Oligonucleotide probes and hybridizing 1 h in 42 ℃.

关 键 词：基因芯片 探针 Poly(dT) 杂交 

分 类 号：Q78[生物学—分子生物学]