维生素D受体mRNA及其蛋白在不同体质指数人群皮下和大网膜脂肪组织中的表达差异  

The mRNA and protein expression differences of vitamin D receptor in subcutaneous and omental adipose tissues of people with different BMI

作　　者：徐家蓉[1] 张力翔[1] 缪珩[1] 

机构地区：[1]南京医科大学第二附属医院内分泌科,江苏南京210011

出　　处：《南京医科大学学报（自然科学版）》2012年第11期1575-1578,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金(2010NJMUZ50)

摘　　要：目的:观察正常、超重和肥胖3组人群皮下和大网膜脂肪组织中维生素D受体(vitamin D receptor,VDR)mRNA及其蛋白表达水平的差异。方法:用半定量RT-PCR方法检测20例正常组、35例超重组、27例肥胖组人群的腹部皮下和大网膜脂肪组织VDR基因的mRNA表达水平;用Western blot方法检测VDR的蛋白表达水平。结果:在皮下脂肪组织中,肥胖组VDRmRNA和蛋白表达水平明显低于正常组和超重组(P<0.05);在大网膜脂肪组织中,超重组VDR mRNA及其蛋白表达增高(P<0.05),肥胖组VDR mRNA及其蛋白表达与超重组相比降低(P<0.05)。结论:肥胖患者脂肪组织中VDR表达水平降低,可能是临床上补充维生素D干预肥胖达不到预期效果的机制之一。Objective：To investigate the mRNA and protein expression differences of vitamin D receptor （VDR） in subcutaneous and omental adipose tissue of people with different BMI. Methods：Twenty people of normal BMI, 35 people of overweight and 27 people of obesity were selected. The mRNA transcription levels of VDR were assayed by RT-PCR, and the VDR protein expression was measured by Western blot. Results： ① In subcutaneous obesity tissue, mRNA transcription and protein expression of VDR was lower in obesity group than in the normal or overweight group （P 〈 0.05） ; ② In omental adipose tissue,mRNA transcription and protein expression of VDR was highest in overweight group among 3 groups （P 〈 0.05）. Compared with the overweight group, mRNA transcription and protein expression of VDR was lower in obesity group（P 〈 0.05）. Conclusion：The VDR expression decreased in the obesity group, which may be one of the mechanisms of the poor clinical outcome of vitamin D intervention therapy.

关 键 词：皮下脂肪 大网膜脂肪 维生素D 维生素D受体 

分 类 号：R589.2[医药卫生—内分泌]