江苏地区健康汉族人T淋巴细胞免疫表型参考值的建立  

Establishment of the referenced immunophenotyping values of T lymphocytes in the healthy Han nationality from Jiangsu province

作　　者：徐晓琴[1] 陈国红[1] 刘晓燕[1] 胡海洋[1] 郭宏雄[1] 丁萍[1] 傅更锋[1] 还锡萍[1] 羊海涛[1] 

机构地区：[1]江苏省疾病预防控制中心性病爱滋病防制所,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2012年第11期1616-1620,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金(BK2009435);江苏省医学重点人才项目(RC2011087)

摘　　要：目的:了解并建立江苏省汉族健康人群外周血T淋巴细胞免疫表型的正常参考值。方法:采用MultiTEST 4色荧光抗体以FACSCalibur流式细胞仪对460例10～64岁江苏省汉族健康人外周静脉血进行标记检测并分析结果,采用SPSS16.0软件包进行统计分析,以百分位数法确定95%参考值范围。结果:男性和女性的CD3+T细胞的百分率、CD3+CD4+T细胞百分率及CD4+/CD8+的比值差异均有统计学意义,表现为女性高于男性;CD3+T细胞的绝对值、CD3+CD8+T细胞百分率及绝对值、CD3+CD4+T细胞绝对值没有性别差异。T淋巴细胞免疫表型各个指标在不同年龄组之间有差异,表现为CD3+T细胞的绝对值、CD3+CD8+T细胞绝对值、CD3+CD4+T细胞绝对值总体来说随年龄增长而有所下降。江苏地区健康汉族人群T淋巴细胞免疫表型参考值范围(P5～P95):CD3+T细胞百分率为50%～79%,CD3+T细胞绝对数为902～2 665个/μl,CD3+CD8+T细胞百分率为15%～37%,CD3+CD8+T细胞绝对值为281～1 106个/μl,CD3+CD4+T细胞百分率为25%～48%,CD3+CD4+T细胞绝对值为453～1 365个/μl,CD4+/CD8+的比值为0.77～2.70。结论:初步建立了江苏省汉族健康人群外周血T淋巴细胞免疫表型参考范围,为获得性免疫缺陷综合征(acquired immunodeficiency syndrome,AIDS)的研究和治疗提供重要的参考依据。Objective：To establish the referenced immunophenotyping values of T lymphocytes in the healthy Han nationality from Jiangsu province. Methods：Total 460 peripheral blood samples were collected from the healthy Hart nationality （aged from 10 to 64 years old）,which were detected by FACSCalibur flow cytometry. The data were analyzed by SPSS16.0. Results：The percentages of CD3^＋ and CD3^＋CD4^＋ T lymphocytes,and CD4^＋/CD8^＋ ratio in peripheral blood were significantly higher in female than in male. There was no significant difference in the absolute number of CD3 ^＋and CD3^ ＋CD4 ^＋ T lymphocytes,and the absolute number and the percentage of CD3 ^＋CD8^＋ T lymphocytes between female and male groups. There were obvious differences in the above indexes during different ages,and the absolute numbers of CD3^＋ ,CD3^ ＋CD8^＋ and CD3^ ＋CD4^＋ T lymphocytes decreased with the age increasing. The referenced immunophenotyping values of T lymphocytes （Ps-P~） in the healthy Han nationality from Jiangsu province were as following. The percentages of CD3^＋,CD3^＋CD8^＋ and CD3^＋CD4^＋ T lymphocytes were 50% to 79%, 15% to 37% and 25% to 48% respectively in peripheral blood. The absolute numbers of CD3^＋, CD3^＋CD8^＋ and CD3^＋CD4＋ T lymphocytes were 902 to 2 665 cells/μl,281 to 1 106 cells/μl and 453 to 1 365 cells/μl respectively. Moreover,the CD4＋/CD8^＋ ratio was 0.77 to 2.70. Conclusion：The referenced immunophenotyping values of T lymphocytes in the healthy Han nationality from Jiangsu province has been established in the study,which provided important reference to researches and treatments relating to HIV/AIDS.

关 键 词：流式细胞仪 T淋巴细胞免疫表型 汉族 参考值 

分 类 号：R392.1[医药卫生—免疫学]