2-KGA混菌发酵过程的结构模型及实验验证  

Structured Modeling and Validation for 2-KGA Mixed Cultivation

作　　者：颜英[1] 谢萍 孙君伟 王涛[1] 丰强强[1] 袁景淇[1] 

机构地区：[1]上海交通大学自动化系系统控制与信息处理教育部重点实验室,上海200240 [2]河北维尔康制药有限公司,石家庄050031

出　　处：《上海交通大学学报》2012年第11期1723-1728,共6页Journal of Shanghai Jiaotong University

基　　金：国家自然科学基金资助项目(60974068);教育部博士点基金(20110073110018)

摘　　要：探讨了古龙酸混菌发酵过程中普通生酮古龙酸菌(Ketogulonicigenium vulgare)和巨大芽孢杆菌(Bacillus megaterium)的交互作用机制.分析了K.vulgare以L-山梨糖为碳源时的代谢途径,建立了K.vulgare的宏观动力学模型,引入了一阶闭环调节器模型来描述糖酵解等酶系的建立过程.该模型描述了K.vulgare中物质流和能量流的平衡关系,据此可解得比碳源消耗速率、比生长速率、比乙酰辅酶A生成速率、比氧消耗速率等关键反应速率.结合生物反应器模型可获得操纵变量与状态变量之间的关系.实验验证了上述模型的有效性.The mechanism of interaction between Ketogulonicigenium vulgate and BacilLus megaterium was investigated for 2-keto-L-gulonic acid （2-KGA） mixed culture. Based on the metabolic pathway analy sis in K. vulgare, a macrokinetic model was constructed, where, a first-order closed-loop regulator model was introduced to describe the induction of the enzyme pool responsible for the growth of K. vulgate at the beginning of the cultivation. The model describes the balance of intracellular substance and energy flow rates. The key reaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetyl-CoA formation rate as well as the specific oxygen uptake rate are obtained with this model. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model such that the relationship between substrate feeding rate and state variables is set up. The experi mental validation results were presented.

关 键 词：酮古龙酸菌 巨大芽孢杆菌 2-KGA混菌发酵 结构模型 代谢途径 

分 类 号：TP273[自动化与计算机技术—检测技术与自动化装置]