紫外-可见分光光度法测定肾蕨中多糖含量的实验性研究  

The Content Determination of Polysaccharide in Nephrolepis auriculata(Linn.) Trimen by UV

作　　者：马雯芳[1,2] 邓慧连[1] 蔡毅[1] 黎理[1] 梁雁[1] 

机构地区：[1]广西中医药大学,广西南宁530001 [2]成都中医药大学,四川成都610075

出　　处：《时珍国医国药》2012年第11期2714-2715,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区食品药品监督管理局面上项目(No.mzy2010005);广西壮族自治区自然科学基金创新研究团队项目(No.2011GXNSFF018006);广西壮族自治区科学研究与技术开发计划项目(No.10100027-1)

摘　　要：目的建立用紫外-可见分光光度法测定肾蕨多糖含量的方法。方法采用苯酚-硫酸法显色反应体系,对显色剂的浓度与用量进行考察,测定肾蕨中多糖的含量。结果以D-无水葡萄糖为对照品,测定波长为489 nm,在1.9～22.8μg·ml-1范围内线性关系良好(r=0.999 0),平均回收率为102.29%(RSD=1.29%,n=6)。结论该方法简便、快捷、准确,可作为肾蕨多糖含量测定的方法。Objective To establish a quantitative method for the determination of polysaecharide in Nephrolepis auriculata （ Linn. ） Trimen by UV - VIS speetrophotometry. Methods The effects of species, concentration, and dosage of chromogenic reagent were studied by the phenol - sulphuric acid method to determine the content of polysaccharide in Nephrolepis auriculata （ Linn. ） Trimen. Results Using rutin as comparison sample, the detection wavelength was set at 489nm. There was good linear re- lationship within 1.9 -22.8 μg .ml^-1 （ r = 0. 9990）. The average recovery rate was 102.29 % （ RSD = 1.29 %, n = 6 ）. Conclusion The method is simple, rapid, accurate and can be used for the quantitative method of Nephrolepis auriculata （ Linn. ） Trimen.

关 键 词：肾蕨多糖 含量测定 显色方法 优化 

分 类 号：R284.2[医药卫生—中药学]