铜绿假单胞菌M2联合小白菜对Pb污染土壤的生物修复  

Bio-remediation of lead polluted soil by Pseudomonas aeruginosa strain M2 combined with pakchoi

作　　者：陈宝[1,2] 徐晓萌[2] 曲娟娟[1] 董蕾[1] 闫立龙[1] 孙兴滨[3] 

机构地区：[1]东北农业大学资源与环境学院寒地黑土资源利用与保护重点试验室,黑龙江哈尔滨150030 [2]浙江大学环境与资源学院污染环境修复与生态健康教育部重点试验室,浙江杭州310058 [3]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《浙江大学学报（农业与生命科学版）》2012年第6期732-740,共9页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：环保公益性行业科研专项资助项目(2010467038)

摘　　要：从Pb污染矿区筛选到1株铜绿假单胞菌 （Pseudomonas aeruginosa） （M2）,采用盆栽试验研究其对Pb污染土壤中小白菜生长、Pb吸收和根际微生物的影响. 结果表明：接种M2菌后小白菜生物量提高20.9 %-31.3 %,地上部Pb含量提高15.0 %-25.4 %,根部Pb含量提高15.6 %-36.8 %;对可培养微生物数量的测定表明,在同样的Pb污染浓度下,接种铜绿假单胞菌的土壤中细菌和真菌数量明显提高,细菌提高17.8 %-29.6 %,真菌提高7.2 %-15.4 %,而放线菌提高不明显;细菌16S rDNA的变性梯度凝胶电泳分析表明,接种铜绿假单胞菌的土壤细菌种类比对照土壤多50.0 %-60.0 %. 结果证明,接种铜绿假单胞菌能够促进小白菜生长,维持土壤细菌多样性,对土壤Pb污染的植物修复有明显的促进作用.As one of the largest mollisol area in the world, the black soil in northeast of China is commonly thought to be fertile and productive. But recently, dozens of blood heavy metal exceeding events were caused by heavy metal polluted agricultural production through food chain. Soil quality of some farmland near a mining site is getting worse and the content of heavy metal has already exceeded the third level of environmental quality standard for soil in China （GB15618-1995）. Crops harvested from these areas had high level of heavy metal and brought a further health problem to human. In order to decrease the adverse effect of heavy metal accumulation in soil, the phytoremediation technology, which is efficient, costless and non-secondary pollution compared with traditional method, has become a hot research point. In many researches, it is a promising way to enhance heavy metal absorption from soil through increasing plants biomass, improving plants resistant to heavy metal and raising the concentration of heavy metal in plants. As many studies proved, inoculation of plant growth-promoting microbes can not only increase plant biomass and nutrition through a number of mechanisms such as nitrogen fixation, production of phytohormones and siderophores, and transformation of nutrient elements, but also can enhance tolerance and accumulation of metals in plants. This experiment was carried out to investigate the effect of bacterium application on the performance of pakchoi （Brassica rapa L. ssp. chinensis） and Pb removal from a contaminated soil. One Pseudomonas aeruginosa strain M2, originally isolated from mining area contaminated with Pb, was examined for its effect on the pakchoi growth, Pb accumulation and rhizospheric microorganism in soil spiked with Pb（NO3）2 in a pot experiment. The strain was cultivated in nutrient medium at 30 ℃ for 48 h, whose cells were collected in the exponential phase by centrifugation at 6 000 r/min for 10 min, and were inoculated to soil pretreated with Pb（NO3）2 with 108

关 键 词：铜绿假单胞菌 PB污染 细菌群落多样性 多聚酶链反应-变性梯度凝胶电泳 生物修复 

分 类 号：Q939.96[生物学—微生物学] X53[环境科学与工程—环境工程]