车前草穗枯病菌分子鉴定  

Molecular Identification of Pathogen of Plantain Head Blight

作　　者：梁艳[1] 涂怀妹 崔朝宇[1] 蒋军喜[1] 

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]江西省安义县农业局,江西安义330500

出　　处：《生物灾害科学》2012年第3期258-260,共3页Biological Disaster Science

基　　金：国家自然科学基金资助项目(30960213)

摘　　要：采用真核生物通用引物ITS1/ITS4,对来自江西省吉安县2个车前草穗枯病菌菌株进行rDNA-ITS区段的PCR扩增,对扩增产物进行测序,结果获得2个长度均为579 nt且序列完全一致的rDNA-ITS序列,该序列与GenBank中当归间座壳菌(Diaporthe angelicae)及其无性态拟茎点霉菌(Phomopsis subordinaria)对应序列的同源性高达99.0%,根据以rDNA-ITS序列同源性大小划分种类的标准,将车前草穗枯病菌鉴定为当归间座壳菌。In the paper, two isolates of pathogen of plantain head blight from Ji＇an county in Jiangxi province were molecularly identified. RDNA-ITS region of each of the two isolates was amplified by PCR, and the PCR products were sequenced. Both of them are 579 nt in length, and they have a consensus sequence. The sequence shares 99.0% homology with sequence of either Diaporthe angelicae or its anamorph Phomopsis subordinaria. Based on the criterion of rDNA-ITS sequence homology in fungus species classification, pathogen of plantain head blight was identified as Diaporthe angelicae.

关 键 词：车前草穗枯病 当归间座壳菌 分子鉴定 

分 类 号：S432.1[农业科学—植物病理学]