改良三袖套法建立大鼠左肺原位移植动物模型  

To establish rat orthotopic left pulmonary allograft transplantation by improving the "cuff-like" vessel anastomosis technique

作　　者：杨天宝[1] 黄国忠[1] 谢金标[1] 

机构地区：[1]福建莆田学院附属医院胸外科,莆田351100

出　　处：《海峡药学》2012年第11期39-41,共3页Strait Pharmaceutical Journal

摘　　要：目的探讨建立更稳定有效的大鼠原位左肺移植模型。方法将40只SD大鼠随机配对,采用三袖套法建立大鼠肺移植模型。结果进行大鼠左肺原位移植正式实验20例,手术平均时间(65±4)min。手术成功率85%。血气、病理学等检查证实成功复制了肺移植缺血再灌注模型。结论该方法操作成功率高,经济实用,高度模仿临床肺移植,可用于肺移植缺血再灌注损伤的实验研究。OBJECTIVE To establish a more stable and effective rat orthotopic left pulmonary allograft transplantation models.METHODS 40 rats were randomly divided into two groups：donors group and recipients group.Pulmonary arteries and veins were anastomosed by traditional cuff technique.Bronchi was reconstructed by stent technique.RESULTS 20 cases of rat orthotopic left pulmonary allograft transplantation was finished successfully.The total time of surgical procedure was（65±4）min.The operation successful rate was 85%.By blood gas and pathologic study we successfully found the typical lung ischemia reperfusion injury model.CONCLUSION It is an economical and successful model in highly imitating clinic lung transplantation,which can be used in lung ischemia reperfusion injury.

关 键 词：肺移植 模型 大鼠 

分 类 号：R969[医药卫生—药理学]