HPLC-RID法测定益母草颗粒中盐酸水苏碱含量  

Content determination of Stachydrine hydrochloride in Herba leonuri particle by HPLC-RID

作　　者：黄强增 

机构地区：[1]福建省福州儿童医院,福州350005

出　　处：《海峡药学》2012年第11期63-65,共3页Strait Pharmaceutical Journal

摘　　要：目的建立益母草颗粒中盐酸水苏碱的含量测定方法。方法采用氨基柱,以乙腈-水(体积比80∶20)为流动相,流速是1mL.min-1,示差折光检测器,柱温40℃。结果盐酸水苏碱浓度在0.2mg.mL-1-1mg.mL-1范围内有良好的线性关系,r=0.9982,回收率为101.5%,RSD为2.5%(n=6)。结论本法专属性强,准确、简便,为益母草颗粒中盐酸水苏碱测定提供新的方法。OBJECTIVE To establish the method for the content determination of Stachydrine hydrochloride in Herba leonuri particle.METHODS The determination was performed on Amino column with acetonitrile-water（volume ratio 80∶20）as mobile phase at the flow rate of 1mL·min-1.Differential refractive index detector（RID） was used,and the column temperature was 40℃.RESULES The linear range of Stachydrine hydrochloride was 0.2mg·mL-1~1mg·mL-1（r=0.9982）with an average recovery of 101.5%（RSD=2.5%,n=6）.CONCLUSION The method is specific,accurate and simple,which can be applied as a new way for the content determination of Stachydrine hydrochloride in Yimucao Keli.

关 键 词：益母草颗粒 高效液相色谱法 示差折光检测器 盐酸水苏碱 

分 类 号：R927.2[医药卫生—药学]