均匀设计法优化五爪金龙提取工艺及不同采收期总黄酮含量测定  

Uniform Design Method for Optimizing the Extraction Technology of Ipomoea Cairica and Content Determination of Flavonoid in Different Harvest Times

作　　者：尹永芹[1] 严优芍[1] 陶曙红[1] 沈志滨[1] 黄世昌[1] 

机构地区：[1]广东药学院中药学院,广东广州510006

出　　处：《中医药导报》2012年第11期14-17,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(81202885);科技部"十二五重大新药创制"项目(2011ZX09102-007-03);广东省科技厅科技计划重点专项(2011A030100013);广东省科技厅科技计划项目(2010B030700044);2010年广东省教育厅高层次人才资助项目

摘　　要：目的:研究五爪金龙总黄酮的最优工艺条件,并对其不同月份总黄酮进行含量测定;方法:通过均匀设计法优选方案,采用索氏加热回流提取法,以芦丁为对照品,采用紫外分光光度仪在406 nm波长处测吸光度,测定五爪金龙中总黄酮的含量;结果:均匀设计实验结果表明,乙醇浓度为90%,提取时间为4 h,料液比为1∶60,颗粒度为60目为最佳反应条件;不同月份总黄酮测得的平均含量为(4.8614±0.1205)%;结论:不同采收期五爪金龙总黄酮含量变化不大,适合大规模长期采收,为五爪金龙的开发利用提取提供参考。Objective： To evaluate the best extraction process for flavonoid of Ipomoea cairica, and content determination of its flavonoid with different harvest times. Methods： Flavonoid of 1. cairica was detected the content determination by uniform design test, using soxhlet extraction method with rutin as reference to measure at 406 nm wavelength. Results： The uniform design experimental results show that ethanol concentration of 90%, the extraction time of 4 hours, the solid-liquid ratio of 1： 60, and particle degree of 60 orders were the best reaction conditions for extraction; the average content of flavonoid with different harvest times were（4.8614＋0.1205）%. Conclusion： There is little change of content of flavonoid from 1. cairica in different harvest times, so that I. cairica is suitabled for large-scale and long-term harvesting, which provide a reference for the development and utilization of I. cairica

关 键 词：五爪金龙 均匀设计法 索氏提取法 总黄酮含量测定 

分 类 号：R284.1[医药卫生—中药学]