从原始生殖细胞分离克隆绵羊胚胎干细胞  

Isolation and cloning of sheep embryonic stem cells from primordial germ cells

作　　者：图雅[1] 卢玲[1] 曹贵方[2] 

机构地区：[1]包头师范学院生物科学与技术学院,内蒙古包头014030 [2]内蒙古农业大学生物工程学院,呼和浩特010018

出　　处：《黑龙江畜牧兽医》2012年第12期1-3,179,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(39960051);内蒙古自治区自然科学项目(990303-2)

摘　　要：为了研究绵羊胚胎干细胞在体外培养过程中的生长行为,试验采用30～45日龄的绵羊胎儿生殖嵴及其周围组织共培养的方式分离克隆胚胎干细胞。结果表明:在不添加任何细胞生长因子的情况下,也能分离克隆到具ES细胞诸多特征的胚胎生殖细胞系,即具有连续传代能力、细胞集落有典型鸟巢状结构、AKP染色阳性等。说明该细胞具有多能性,类似ES细胞。To research the growth behavior of sheep embryonic stem cells （ESCs） cultured in vitro, the ESCs wereisolated and cloned from the genital ridge and its surrounding tissues of seep embryos （ 30-35 days old） by co - culture method. The results showed that without any growth factor, the embryonic germ cell line could be obtained by the isolation and clone, which had many characteristics of ES ceils, such as having the ability to continuously passage, nest - like ES cells colonies , and positive AKP staining. The results indicate that the cells have versatility, which are similar to ES cells.

关 键 词：原始生殖细胞 胚胎干细胞 绵羊 

分 类 号：S826[农业科学—畜牧学]