高效液相色谱法测定土壤和烟叶中精喹禾灵的残留量  

Determination of quizafop-p-ethyl residue in soil and tobacco by high performance liquid chromatography

作　　者：丁春霞[1,2] 龚道新[2,3] 肖浩[2,3] 任颖俊[2,3] 王佳[1] 彭文军[1] 唐文彬[1] 

机构地区：[1]湖南农业大学理学院,湖南长沙410128 [2]湖南农业大学资源环境学院,湖南长沙410128 [3]湖南农业大学农业环境保护研究所,湖南长沙410128

出　　处：《应用化工》2012年第11期1999-2001,2003,共4页Applied Chemical Industry

基　　金：农业部农残试验基金项目(2010H216)

摘　　要：建立了测定土壤和烟叶中精喹禾灵残留量的高效液相色谱分析方法。土壤和烟叶样品中精喹禾灵用丙酮提取,经弗罗里硅土净化后,采用XB-C18柱(150 mm×4.6 mm,5.0μm),以甲醇-水(80:20,v:v)为流动相,流速为0.6 mL/min,柱温为25℃,检测波长为236 nm,采用外标法定量。精喹禾灵的浓度在0.05～5.00 mg/L范围内时,其线性关系良好(R2=0.997 4),回收率在85.47%～106.63%,相对标准偏差在0.71%～4.26%。该方法操作简便,灵敏度、准确度和精密度高,能满足农药残留量分析与检测技术的要求。An analytical method was established to determine the residue of quizafop-p-ethyl in soil and tobacco by high performance liquid chromatography. The residues in soil and tobacco was extracted with acetone,and the extract was cleaned up by a Florisil chromatography column. The separation was per- formed on a C18 column（ 150 mm ×4.6 mm,5.0 μm）at 25 ℃ by HPLC with an ultraviolet dector（UVD） at 236 nm,and methanol-water（80：20,v：v） as the mobile phase at a flow rate of 0. 6 mL/min. The quan- tification was performed by external standard. The calibration curve was linear （ R2 = 0. 997 4 ） between the peak area and the concentration when its concentration ranged from 0.05 mg/L to 5.00 mg/L. The average recoveries were between 85.47% - 106.63% with its relative standard deviations of 0.71% ~ 4.26%. This method was characterized by simplicity, sensitivity and accuracy, and could meet the techno- logical acquirement of pesticide residue analysis.

关 键 词：精喹禾灵 高效液相色谱 残留量 测定 烟田 

分 类 号：TQ450.2[化学工程—农药化工]