四种全自动尿沉渣分析仪对尿液中红细胞和白细胞的检测性能研究  

Performances of Four Automated Urine Sediment Analyzers in Detecting Urine Red Blood Cells and White Blood Cells

作　　者：梁骑[1] 李君安[1] 王东生[1] 万松[1] 费中海[1] 吴昊[2] 焦艳梅[2] 唐中[1] 

机构地区：[1]川北医学院附属医院检验科,四川省南充市637100 [2]首都医科大学附属北京佑安医院感染中心

出　　处：《中国全科医学》2012年第32期3737-3742,共6页Chinese General Practice

基　　金：国家自然科学基金(30872226);国家十二五重大科技专项资助项目(2012ZX10001-003);国家十二五重大科技专项资助项目(2012ZX10001-006)

摘　　要：目的评价FUS-200、IQ-200、AVE-764B、UF-500i四种尿沉渣分析仪对尿液中红细胞(RBC)、白细胞(WBC)的检测性能。方法对FUS-200、IQ-200、AVE-764B、UF-500i四种尿沉渣分析仪检测尿液中RBC、WBC的批内精密度、携带污染率、线性范围、干扰实验进行评价。选择100例阳性尿样本分别用以上四种尿沉渣分析仪检测RBC、WBC,并将检测结果与人工镜检的结果进行对比分析。结果 FUS-200、IQ-200、AVE-764B、UF-500i四种尿沉渣分析仪检测尿液中RBC的批内精密度分别为7.0%、5.8%、6.3%、3.2%,携带污染率分别为0、0.04%、0、0.09%;检测WBC的批内精密度分别为9.8%、8.2%、4.9%、5.9%,携带污染率分别为0、0.41%、0、0.12%。当RBC在0～15 000个/μl、WBC在0～10 000个/μl范围内时,四种尿沉渣分析仪的测定值与理论值的线性相关系数R2均>0.99。UF-500i、FUS-200修饰后、IQ-200修饰后、AVE-764B修饰后的RBC及WBC检测结果与人工镜检的结果比较,差异均无统计学意义(P>0.05),真菌和结晶会干扰四种尿沉渣分析仪对RBC和WBC的检测结果。结论 FUS-200、IQ-200、AVE-764B、UF-500i四种尿沉渣分析仪检测尿液RBC和WBC的精密度高、线性好、携带污染率低,可用于临床样本的初筛,但不能完全取代人工镜检。Objective To analyze the performances of four automated urine sediment analyzers （FUS -200, IQ - 200, AVE -764B, and UF- 500i） in detecting urine red blood cells （RBC） and white blood cells （WBC）. Methods The within - run precision, linearity, carryover rate, and anti - interference performance of four automated urine sediment analyzers （FUS -200, IQ -200, AVE -764B, and UF- 500i） in detecting urine RBC and WBC were tested. WBC and RBC were de- tected using these four analyzers in 100 patients with positive urine samples, and the results were compared with those of manual microscopy. Results The within - run CVs for WBC counting and RBC counting respectively were 7.0% and 9. 8% for FUS - 200, 5.8% and 8.2% for IQ - 200, 6. 3% and 4. 9% for AVE - 764B, and 3.2% and 5.9% for the UF - 500i. The ear- ryover rates for RBCs and WBCs were 0. 04% and 0.41%, respectively, for IQ - 200, O. 09% and 0. 12% for UF - 500i, 0 and 0 for AVE-764B, and 0 and 0 for FUS-200. All these four automated urine sediment analyzers showed a good linearity （R2 〉0. 99） for RBC with 0 - 15 000/μl range and WBC with 0 - 10 000/1μ1 range. The results of RBC and WBC counting showed no significant differences between these four analyzersand and manual microscopy （ P 〉 0. 05 ）. However, the detection of RBC/WBC by these four analyzers were interfered by fungi and crystals. Conclusion All these four automated urine sediment analyzers have high precision, good linearity, and low carryover rate in detecting RBC and WBC in urine sediment. They can be used for the screening of clinical specimens, although they still can not replace the microscope examination.

关 键 词：UF-500I IQ-200 AVE-764B FUS-200 红细胞 白细胞 尿沉渣分析仪 

分 类 号：R446.121[医药卫生—诊断学] R319.9[医药卫生—临床医学]