中性粒细胞吞噬功能试验预染色法的探讨  

Study on pre-staining method of neutrophil phagocytosis function test

作　　者：王地英[1] 刘玲霞[1] 

机构地区：[1]苏州大学附属常州肿瘤医院检验科,江苏常州213001

出　　处：《检验医学与临床》2012年第23期2960-2961,2963,共3页Laboratory Medicine and Clinic

摘　　要：目的建立预染色法中性粒细胞吞噬功能试验,探讨血细胞计数仪进行测定的可行性。方法通过对吞噬前细菌进行预染色,使细菌容易辨认而提高试验准确性。观察不同时间中性粒细胞吞噬试验的形态学变化、细胞数量变化及不同的时间血细胞计数仪测定中性粒细胞的结果,并进行比较分析,确定测定时间。结果不同孵育时间中性粒细胞吞噬细菌的形态不同,细菌清晰、易认;1h后显微镜法的中性粒细胞吞噬率健康对照组(58.4±20.5)%,2型糖尿病组(48.5±19.8)%,肿瘤组(51.1±21.4)%;仪器法的中性粒细胞吞噬率,健康对照组(55.6±17.0)%,2型糖尿病组(46.4±15.6)%,肿瘤组(47.5±16.1)%。结论显微镜计数法和血细胞分析仪法的结果密切相关,可以为临床应用。Objective To establish the Pre-staining method of neutrophil phagocytosis function test,and to explore the feasibility of determination by blood count instrument. Methods Pre-stained of bacteria of phagocytosis was used to identify the bacteria easily and improve the test accuracy. The morphological changes of neutrophils , cell number changes and the results of neutrophil from blood counter analyzer were observed in different time and anaiyzed comparatively for making the time of determination. Results Passing different incubation time, neutrophils morphology were different ithe bacteria were clear,and it was easy to be recognized. After one hour, microscopy neutrophils phagocytosis rates were normal control group （58.4±20.5） %, type 2 diabetes group（48.5 ± 19.8）%, tumor group（51.1 ± 21.4）%. Instrument neutrophils phagocytosis rates were normal control group（55.6 ± 17.0）%, type 2 diabetes group（46.4±15.6）% ,tumor group（47.5±16.1）%. Conclusion After incubation 1 hour, blood count of instrument neutrophils phagocytosis rate is closely related（r=0. 989） with microscope notation,which can applied in clinic.

关 键 词：中性粒细胞 吞噬功能 预染色 

分 类 号：R563[医药卫生—呼吸系统]