二长棘犁齿鲷线粒体DNA控制区结构和进化  

Structure and evolution of mitochondrial control regions in Evynnis cardinalis

作　　者：苏天凤[1] 江世贵[1] 马之明[1] 李夏[1] 

机构地区：[1]中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《南方水产科学》2012年第6期1-8,共8页South China Fisheries Science

基　　金：广东省科技计划项目(2010B060200022)

摘　　要：测定了二长棘犁齿鲷(Evynnis cardinalis)线粒体控制区全序列,并结合从GenBank中下载的9种鲷科鱼类的相应序列,采用Clustal X对控制区结构进行了分析,识别了相应的保守序列,包括终止相关序列ETAS、中央保守区的CSB-F、CSB-E、CSB-E、CSB-C、CSB-B和CSB-A序列,以及保守序列区的CSB1、CSB2和CSB3序列。以副鲈(Paralabrax clathratus)和条斑星鲽(Verasper moseri)为外群,用最大简约法(MP)和邻接法(NJ)构建了系统发育树。结果显示鲷科鱼类构成一个单系类群,二长棘犁齿鲷应该归入赤鲷属,建议有必要对犁齿鲷属和赤鲷属的分类地位重新评估。We determined the complete sequences of the mitochondrial DNA control regions in Evynnis cardinalis using long polymer- ase chain reaction （PCR）, and aligned them by Clustal X with 9 related Sparidae sequences in GenBank. Several conserved sequence blocks were identified in the mitochondrial DNA control regions, including extended terminal associated sequences （ETAS）, blocks of central domain （CSB-F, CSB-E, CSB-D, CSB-C, CSB-B, CSB-A） and conserved sequence blocks （CSB1, CSB2, CSB3）. Tak- ing Paralabrax clathratus and Verasper moseri as outgroups, the molecular phylogenetic trees of Sparidae were constructed based on neighbour-joining and maximum-parsimony methods, which reveal that Sparidae is a monophyletic group, and E. cardinalis should be grouped with Pagrus. Therefore, it is necessary to reevaluate the taxonomic status of Evynnis and Pagrus.

关 键 词：二长棘犁齿鲷 控制区 结构 系统关系 

分 类 号：Q951[生物学—动物学] S917.4[农业科学—水产科学]