基于micro CT扫描技术的鲤骨骼和显微结构分析  

Study on bone and microstructure of common carp using micro CT scanning

作　　者：张宁[1] 苏胜彦[1,2] 董在杰[1,2] 马庆男[2] 袁新华[1,2] 

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081

出　　处：《南方水产科学》2012年第6期44-49,共6页South China Fisheries Science

基　　金：现代农业产业技术体系建设专项资金项目(CARS-46);中央级公益性科研院所基本科研业务费专项资金项目(2009JBFB01);江苏省自然基金项目(BK2010164);国家科技支撑计划项目"大宗淡水主养鱼类新品种选育"(2012BAD26B02)

摘　　要：了解活体鲤(Cyprinus carpio)骨结构、形态、连接、空间分布以及骨骼矿物质组成对鲤的选育、行为学研究以及肉品加工有重要的意义。X射线显微CT技术(micro CT)对样品没有破坏,可实现活体检测,并能获取活体任意形态的结构参数。因此,文章通过这项技术来研究活体鲤的骨骼状况。结果显示,脊椎骨与头、尾相连,胸鳍位于头骨后,背鳍、腹鳍和尾鳍与整个脊椎骨并不相连。骨体积分数和骨小梁厚度在腹鳍较高,头尾较低;骨表面积体积比、骨小梁数量和骨小梁间隔在头部较高,腹鳍较低;骨矿物质含量、骨矿物质密度和组织矿物质含量在背鳍最高;骨体积分数在腹鳍最高,胸鳍、背鳍和尾鳍次之,头尾较低。骨小梁厚度、矿物质含量等在背鳍、腹鳍部位较高,这可能与鲤的运动有关,而头骨表面积体积比、骨小梁数量和骨小梁间隔较高可能与头部的复杂结构密切相关。It is important to understand the microstructure, morphology, connection, spatial distribution and mineral constitution in vivo of bone of common carp （ Cyprinus carpio） for its breeding, behavior research and fish processing. X-ray microscopic CT technolo- gy （ micro CT） does not destroy the samples and is easy for bio-assay to measure the structure parameters of arbitrary shape. Thus, we apply micro CT to scan common carp＇s bone and find that： vertebrae are joined with head and tail; pectoral fin is located behind head; dorsal fin, ventral fin and caudal fin are separated from vertebrae; ventral fin has higher while head has lower BV/TV and Tb. Th; head has higher BS/BV, Tb. N and Tb. Sp; dorsal bone has higher bone mineral content, bone density （BMD）, tissue mineral content, while abdomen has higher bone volume fraction （BVF） , next are pectoral fin, dorsal fin, head and tail. Dorsal fin and ventral fin have higher Tb. Th and mineral content, which may be caused by movement of common carp, and head＇s higher BS/BV, Tb. N and Tb. Sp may be related with head＇s complex structure.

关 键 词：鲤 MICRO CT 骨 显微结构 

分 类 号：S911[农业科学—水产科学]