水稻稻瘟病抗性QTL的定位分析  

Mapping QTLs for rice blast resistance in DH line derived from Muwanggu and E'wan8

作　　者：杨小林[1,2] 戚华雄[3] 殷得所[3] 曾凡松[2] 张舒[2] 喻大昭[2] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]农作物重大病虫草害可持续控制湖北省重点实验室,湖北省农业科学院植保土肥所,武汉430064 [3]湖北省农业科学院粮食作物研究所,武汉430064

出　　处：《植物病理学报》2012年第6期600-607,共8页Acta Phytopathologica Sinica

基　　金：国家科技计划项目(2012BAD19B03)

摘　　要：稻瘟病是危害水稻最严重的病害之一。以抗稻瘟病的云南省地方品种魔王谷(MWG)和感稻瘟病的湖北省审定品种鄂晚8号(EW8)为亲本材料,构建双单倍体分离群体(DH)。利用22个菌株对亲本材料MWG/EW8进行致病性鉴定,从中筛选到5个毒性不同的鉴别菌株用于考察DH群体的稻瘟病抗性,构建包含120对SSR标记的分子遗传连锁图进行数量性状位点(QTL)的分析,鉴定出3个抗性QTL,均位于第6染色体长臂RM541附近,3个QTL对抗病表型的贡献率介于7.7%～15.2%之间,3个QTL的抗病等位基因均源自亲本MWG。Rice blast is one of the destructive diseases in rice. A doubled haploid （DH） population derived from a cross between Mowanggu, a famous resistant cultivar to rice blast from Yun＇ nan Province, and E＇ wanS, a susceptible cultivar from Hubei Province. 5 isolates were screened among 22 isolates, which could test insusceptible/susceptible reaction in Mowanggu/E＇ wanS, respectively. The resistance to rice blast of the DH population was monitored with the 5 isolates. Quantitative trait locus （QTL） was detected using a genetic map including 120 SSR. 3 QTLs were identified and linked to SSR marker RM541 in rice chromosome 6 with variance explained between 7.7% -15.2%. The 3 resistance QTLs were donated by Mowanggu.

关 键 词：水稻 稻瘟病 DH群体 QTL 

分 类 号：S432.45[农业科学—植物病理学]