桉树溃疡病病原菌的鉴定  

Identification of the pathogen causing canker in eucalyptus

作　　者：余磊[1] 郑建芬[2] 王荔芳[1] 赵建荣[1] 徐胜光[1] 高东[2] 黄琼[2] 

机构地区：[1]昆明学院农学院/云南省高校都市型现代农业工程研究中心,昆明650214 [2]云南农业大学植物保护学院/农业生物多样性应用技术国家工程研究中心,昆明650201

出　　处：《植物病理学报》2012年第6期633-636,共4页Acta Phytopathologica Sinica

基　　金：昆明学院科学研究基金(YJL11002);云南省教育厅科学研究基金(2011Z032);教育部博士点基金(20095302110003)

摘　　要：桉树（Eucalyptus ssp．）是世界人工造林三大速生树种（桉树、杨树与松树）之一，也是我国南方营造速生丰产林的主要造林树种。目前，全国桉树人工林面积已超过200万hm2。随着桉树人工纯林的面积扩大，桉树病害也日趋加重，严重影响和制约桉树产业的发展。2008年至今，在云南省石林县和红河州桉树种植区发生一种病害，主要为害嫩枝、枝条和主干，发病初期，感病枝条和主干上产生红褐色圆或椭圆形坏死斑，以后逐渐扩大成椭圆或不规则溃疡斑，病变扩展到木质部后，常造成枝条生长畸形，易引起刮风断裂。The identification of the pathogens isolated from the canker of eucalyptus （Eucalyptus grandis） were carded out. Seventeen diseased eucalyptus samples were observed in Shilin and Honghe in Yunnan Province （ south-western China）. Seventeen isolates of possible pathogenic fungi were isolated from trunks and branches of infected eucalyptus plants. Bases on the pathogenicity experiment, the results showed that YNE1- YNE17 were the pathogens of eucalyptus （E. grandis ） canker disease. The morphological identification showed that YNE1-YNE17 was identified as Botryosphaeria dothidea. By analysis of the rDNA internal tran- scribed spacer region （ ITS1-5.8S-ITS2 ）. BLAST searches at GenBank showed a high identity with reference sequences （99% - 100% ）. Representative sequences of both regions were deposited to GenBank （ Accession No. FJ517657, FJ517658 ）. Morphological and molecular identification confirmed this species as B. do- thidea. B. dothidea was first reported as the pathogen of eucalyptus canker and shoot blight disease in China.

关 键 词：桉树人工林 溃疡病 病原菌 鉴定 枝条生长 速生丰产林 速生树种 人工造林 

分 类 号：S792.39[农业科学—林木遗传育种]