植物病害定点定量式接种器的研制与应用  

Application test on an in-situ quantitative inoculator

作　　者：赵云和[1] 曹克强[1] 刘如香[1] 王娟[1] 夏丽琴[1] 张瑜[1] 王树桐[1] 

机构地区：[1]河北农业大学植物保护学院,保定071001

出　　处：《植物病理学报》2012年第6期655-658,共4页Acta Phytopathologica Sinica

基　　金：国家现代苹果产业技术体系建设岗位专家专项(nycytx-08-04-01);公益性农业行业科技项目(200903004)

摘　　要：在植物病理学研究中，经常遇到植物病害接种问题。对于植物病原真菌来说，常用的接种方法有喷雾法、喷粉法、沉降塔定量接种法；对于细菌病害来说，常用喷雾法。植物病原菌对植物的侵染往往需要一定的水分，这些水很容易在植物叶面移动，因而，无论是喷雾法还是喷粉法，很难实现定量接种。孢子沉降法虽然能够使真菌孢子在一定的空间内比较均匀地沉降，但是对于受体植物的叶片来说，很难保证每张叶片都保持一定的接受角度；沉降塔式接种只能用于室内研究，购买沉降塔也相对比较昂贵。In order to make inoculation to the test plants, a new type of quantitative inoculator was designed and manufactured. It was composed of two parts, a plastic grip and a plastic capsule. The inoculator was small, light and portable. Based on field tests of Valsa mali on apple leaves and Phytophthora infestans on po- tato leaves, inoculation could be easily made on fixed leaf area with quantitative inoculum with high efficien- cy. The inoculator could be used in many cases, such as the identification of plant disease resistance, plant disease epidemiology and bio-assay tests of fungicides.

关 键 词：植物病害 接种器 应用 定点 植物病原真菌 真菌孢子 植物病理学 植物病原菌 

分 类 号：S432[农业科学—植物病理学]