油菜素内酯对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响  

Effects of brassinolide on the isozyme expressions of aerobic respiration enzymes in cucumber seedling roots under hypoxia stress

作　　者：陆晓民[1,2] 孙锦[1] 郭世荣[1] 何立中[1] 王丽萍[1] 阳燕娟[1] 

机构地区：[1]南京农业大学园艺学院、农业部南方蔬菜遗传改良重点开放实验室,南京210095 [2]安徽科技学院,安徽凤阳233100

出　　处：《生态学杂志》2012年第12期3070-3074,共5页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30871736);国家重点基础研究发展计划项目(2009CB119000);现代农业产业技术体系建设专项(CARS-25-C-03);江苏省农业三项工程项目(SX(2011)289);江苏高校优势学科建设工程项目资助

摘　　要：探讨了24-表油菜素内酯(EBR)对低氧胁迫黄瓜幼苗根系有氧呼吸同工酶表达的影响。结果表明:低氧胁迫增强了异柠檬酸脱氢酶(IDH)、琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)及苹果酸酶(ME)同工酶的表达,并产生了一些新的条带;低氧下施用10-3mg·L-1的外源EBR处理后6、9d时IDH、MDH同工酶的表达分别比单纯低氧处理提高了52.8%、13.6%及39.1%、11.3%,ME同工酶的表达在处理3d时比单纯低氧处理提高了11.6%,SDH同工酶表达6、9d时则分别比单纯低氧处理下降了42.9%和36.1%。可见,低氧胁迫下营养液添加EBR可调节黄瓜根系IDH、SDH、MDH及ME同工酶的表达,进而有利于缓解低氧胁迫对黄瓜幼苗根系的伤害。A hydroponic experiment was conducted to study the effects of 24-epibrassinolide （EBR） on the isozyme expressions of aerobic respiration enzymes in cucumber seedling roots un der hypoxia stress. Hypoxia stress promoted the isozyme expressions of root isocitrate dehydrogen- ase （IDH）, succinate dehydrogenase （SDH）, malate dehydrogenase （MDH）, and malie en zyme （ME） , and produced several new bands. Applying 10-3 mg · L-1 of EBR under hypoxia stress increased the isozyme expressions of IDH and MDH on day 6 and day 9 by 52.8% and 13.6%0 , and 39.1% and 11.3% , respectively and the isozyme expressions of ME on day 3 by 11.6% , but decreased the isozyme expressions of SDH on day 6 and day 9 by 42.9% and 36. 1% , respectively, as compared with those under hypoxia stress alone. These results indicated that applying EBR under hypoxia stress could regulate the isozyme expressions of IDH, SDH, MDH, and ME in cucumber seedling roots, and thereby, alleviate the damage of hypoxia stress on the seedling roots.

关 键 词：黄瓜 低氧胁迫 24-表油菜素内酯 有氧呼吸 同工酶 

分 类 号：S642.2[农业科学—蔬菜学]