通过花粉管途径将BYDV-GPV株系缺失复制酶基因导入小麦  

Transformation of Wheat with Defective Replicase Gene of BYDV-GPV via Pollen Tube Pathway

作　　者：吴茂森 田苗英 陈彩层 张文蔚 成卓敏[1] 

机构地区：[1]中国农科院植保所植物病虫害生物学国家重点实验室,北京100094

出　　处：《农业生物技术学报》2000年第2期125-127,共3页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划资助!BH-02-05-01

摘　　要：大麦黄矮病毒（Barlearyellowdwarfvirus,BYDV）是小麦上发生的最重要的病毒，GPV株系是我国小麦黄矮病发生的主流株系。本研究将GPV株系3′端缺失的复制酶基因序列构建成用于转化小麦的植物表达载体，通过花粉管途径将目的基因转入小麦之中。T0及T1代的分子检测结果表明，目的基因已经整合到了小麦的染色体之中。获得的2个转基因系的T1代苗在温室中的初步抗病检测显示，转基因后代对GPV株系具有一定抗性。Barley yellow dwarf virus is the most important virus of wheat. BYDV-GPV strain is one of the main strains in China.In this research, the plant expression plasmid was constructed using the ORF2 gene of GPV in which 3' end was deleted (ORF2-D).Wheat (Jinmai 47) was transformed with the consthected plasmid via Pollen tube pathway. The results of molecular analysis showed that the ORF2-D had been integrated into the wheat genome. T1 plants of the transgenic lines (2 and 3) were indicated resistance to GPV to some extent.

关 键 词：花粉管途径 BYDV-GPV复制酶基因 小麦转化 

分 类 号：Q943[生物学—植物学] S512.1[农业科学—作物学]