核酸类似物诱导蚕豆高频率类减数分裂  

Somatic Meiosis-like Reduction Induced at High Frequency by Nucleotide Analogues in Vicia faba

作　　者：张丽华[1] 陈一华[1] 陈正华[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《农业生物技术学报》2000年第2期147-150,共4页Journal of Agricultural Biotechnology

摘　　要：建立了用咖啡因、尿嘧啶处理蚕豆（Viciafaba）根尖细胞，诱导高频率体细胞类减数分裂的体系。筛选出最佳药物处理浓度及最佳恢复时间，咖啡因处理过程中减数分裂的频率最高达54.0％，尿嘧啶处理类减数分裂频率最高达35.7％。观察体细胞类减数分裂的全过程，发现体细胞染色体减数存在着两种类型，其一为染色体减数分组，多数情况下染色体分为两个组，但未见纺锤丝的作用；另一类型为与配子发生过程相似的减数分裂，出现染色体配对、交叉的图样。这一体系的建立对单倍体的诱导及细胞工程育种有着重要意义。Root tip cells of Vnd faba were treared by caffeine and uracil to induce high frequency of somatic meiosis-like reduction.Optimum treatments including the concenttalion of the chemicals and the time of restoration were screened out. The highest frequency of somatic meiosis'like reduction approached up to 54.0 % and 35.7 % for caffeine and uracil treatment respectively. The process ofsomatic meiosis-like reduction was observed, which existed in tWo types. one was chromosome reductional grouping, in which chromosomes in a cell usually separated into two groups without the role of spindle fibers. The other type was somatic meiosis, which was analogous to meiosis in gamatogenesis and characterized by pairing and chiasmata of chromosomes. Establishment of this inducing system has a profound meaning in the study of producing hgnIoid cells as well as in cell engineering breeding.

关 键 词：蚕豆 核酸类似物 类减数分裂 

分 类 号：Q942[生物学—植物学] S643.6[农业科学—蔬菜学]