蛋白酶体抑制剂MG-132对柯萨奇B3病毒性心肌炎小鼠的作用研究  

Effect of MG-132 in mice with acute viral myocarditis

作　　者：张新民[1] 李岳春[1] 陈鹏[1] 

机构地区：[1]温州医学院附属第二医院心内科

出　　处：《中国临床药理学与治疗学》2012年第11期1229-1232,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究泛素蛋白酶体抑制剂MG-132对柯萨奇B3(CVB3)病毒性心肌炎小鼠的作用,探讨泛素蛋白酶体系统在病毒性心肌炎发病学中的作用机制。方法:随机将80只雄性BALB/C小鼠分为4组(n=20):正常对照组、心肌炎组、心肌炎+处理组、正常+处理组。腹腔接种CVB3诱发急性心肌炎,次日腹腔注射MG-132,0.75mg/kg;连续给药7d,对照组腹腔注射PBS。第8天小鼠取材,观察心脏病理变化,测定心肌CVB3病毒复制及血清肌钙蛋白、脑钠肽水平。结果:MG-132显著减轻心肌炎小鼠心脏病理损伤,显著降低心脏重量/身体重量比值,MG-132干预后第8天小鼠血清肌钙蛋白、脑钠肽水平显著降低,同时荧光定量PCR显示CVB3mR-NA复制水平显著降低。结论:MG-132通过抑制CVB3病毒复制,显著减轻心肌炎小鼠心脏病理损伤,起到保护心肌作用。AIM： To determine the ettect ot proteasome inhibitor MG-132 in mice with acute viral myocarditis induced by eoxsackievirus B3 virus （CVB3） infection. METHODS： BALB/C mice were intraperitoneally inoculated with CVB3 to induce myocarditis. From 24 h after in fection, MG-132 was administered at a dose of 0.75 mg/kg for 7 d continuously by intraperito- neal injection. Normal controls were treated with same volume of PBS. Then, the myocardial histopathological changes, HW/BW ratio, ex pression of myocardial CVB3 mRNA and serum cTn I and BNP were determined. RESULTS： MG-132 significantly attenuated myocardial le-sions and decreased HW/BW ratio. It also re markably decreased serum cTn I and BNP, down-regulated CVB3 mRNA expression com- pared with myocarditis controls. CONCLU SION： MG-132 protects against CVB3 induced viralmyocarditis in mice. MG-132 exerts its ben- eficial effect by downregulating CVB3 mRNA expression probably.

关 键 词：泛素蛋白酶体系统 MG-132 小鼠 心肌炎 病毒复制 

分 类 号：R965.2[医药卫生—药理学]