苹果蠹蛾线粒体DNA COⅠ基因克隆与序列分析  

Cloning and sequencing analysis of the codling moth Cydia pomonella CO Ⅰ gene

作　　者：冯宏祖[1,2] 王兰[1] 郭文超[3] 曹玉[1] 杨明禄[1] 刘慧敏[1] 许建军[3] 

机构地区：[1]塔里木大学植物科学学院,阿拉尔市843300 [2]塔里木盆地生物资源保护利用重点实验室,阿拉尔市843300 [3]新疆农业科学院植物保护研究所,乌鲁木齐市830091

出　　处：《应用昆虫学报》2012年第6期1503-1507,共5页Chinese Journal of Applied Entomology

基　　金：公益性行业(农业)科研专项(200903042);973计划课题(2009CB119204)

摘　　要：本研究采集新疆阿拉尔地区苹果蠹蛾Cydia pomonella(L.)幼虫,对其线粒体DNA细胞色素氧化酶Ⅰ亚基(COⅠ)基因进行了扩增、克隆和测序,并对COⅠ序列进行了分析。结果显示:苹果蠹蛾DNA扩增出的COⅠ基因序列片段长度为709bp,序列中A+T含量极高,占68.7%,而G+C的含量只有31.3%。经基因序列比对,与其它几种食心虫的同源性为85.4%～88.1%,遗传距离为0.130～0.162;采用NJ法构建了卷蛾科系统树,所得的聚类结果与传统的分类结果基本一致。本研究结果为苹果蠹蛾快速鉴定的DNA条形码技术研究提供重要基础。The mitochondfial COI gene fragment of codling moth Cydia pomonella （L.） specimens from the Alar area of Xinjiang was amplified via PCR and the PCR products were purified, cloned and sequenced. 709 bp nucleotide sequences were obtained with a very high A ＋T content （68.7%） and relatively low G ＋ C content （31.3%）. Comparing the COI sequence of the codling moth with that of several other moth species, the homologies of nucleotides ranged from 85.4% to 88.1% and the genetic distances ranged from 0. 130 to 0. 162. Reconstructing the molecular phylogenetic tree of the Tortricidae with the Neighbor-Joining （NJ） method produced a result in accord with the traditional morphological classification. The results of this study provide an important basis for the rapid identification of C. pomoneUa with DNA barcode technology.

关 键 词：苹果蠹蛾 COⅠ基因 克隆 序列分析 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]