微流控芯片技术用于三种小蠹COⅠ-COⅡ区的PCR-RFLP分析  

Analysis of PCR-RFLP data on the CO Ⅰ-CO Ⅱ gene of three bark beetle species (Coleoptera:Scolytidae) by microfluidics

作　　者：张龙霞[1] 李惠萍 裴雁曦[1] 

机构地区：[1]山西大学生命科学学院,太原030006 [2]山西出入境检验检疫局,太原030024

出　　处：《应用昆虫学报》2012年第6期1721-1725,共5页Chinese Journal of Applied Entomology

基　　金：国家质检总局风险分析专项和重大植物疫情监测和风险预警关键技术研究(2008IK264)

摘　　要：本研究运用PCR-RFLP对红脂大小蠹Dendroctonus valens LeConte、长毛干小蠹Hylurgops longipilis Reiher和六齿小蠹Ips acuminatus Gyllenhal mtDNA的COⅠ-COⅡ基因进行扩增。通过酶切位点的分析比较,筛选特异性的内切酶,结合微流控芯片技术对酶切产物进行快速检测。建立3种小蠹的准确、快速区分鉴定方法。PCR-RFLP was used to amplify and analyze the CO I -COII genes in the mtDNA of Dendroctonus valens LeConte, Hylurgops longipilis Reiher and lps acuminatus Gyllenhal. Specific restriction enzymes were screened by comparison of restriction recognition sites. The amplified gene fragments could be analyzed quickly by microfluidics following enzyme digestion. The results indicate that this technique can accurately and rapidly identify these three beetle species.

关 键 词：微流控芯片技术 红脂大小蠹 长毛干小蠹 六齿小蠹 PCR—RFLP 

分 类 号：S763.3[农业科学—森林保护学]