农抗702对植物病原真菌的抑制效果及抑菌机理  

Inhibition effect of Ag-antibiotic 702 on plant pathogenic fungi and related mechanisms

作　　者：魏赛金[1,2] 杜亚楠[1] 倪国荣[2] 张慧雯[1] 涂国全[1] 潘晓华[2] 

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045 [2]江西农业大学作物生理生态与遗传育种教育部重点实验室/江西省作物生理生态与遗传育种重点实验室,南昌330045

出　　处：《应用生态学报》2012年第12期3435-3440,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30960011);国家自然科学基金面上项目(31071724);江西省自然科学基金项目(2009GZN0030;2010GZN0037)资助

摘　　要：为探索农抗702在植物病害生物防治中的实际应用价值及对病原真菌的作用机理,采用生长速率法测定其抑菌谱,并以水稻纹枯病菌为研究对象,测定其作用下菌丝的电解质渗漏、蛋白质、核酸、Mg2+、K+含量的变化,及其对细胞膜麦角甾醇生物合成和水稻纹枯病菌超显微结构的影响.结果表明:农抗702对供试13种病原真菌均有较强的抑制作用,其中对油菜菌核病菌的作用最强,EC50为0.23μg·mL-1;农抗702作用水稻纹枯病菌菌体后相对电导率增加72.2%;蛋白质、核酸、Mg2+、K+等胞内物质均发生了不同程度的渗漏;细胞麦角甾醇含量降低了92.0%;细胞膜轮廓不清、破损;细胞器严重损伤,有空泡化.抑制麦角甾醇合成、造成真菌细胞膜渗透增加是农抗702抑制真菌的主要机理.To explore the practical application value and action mechanisms of Ag-antibiotic 702 against pathogenic fungi, the inhibition spectrum of Ag-antibiotic 702 was studied by measuring the mycelium growth rate of pathogenic fungi, and the effects of Ag-antibiotic 702 on the membrane permeability of Rhizoctonia solani, a typical pathogenic fungus, were investigated, with the variations of myeelium electrolyte leakage and protein, nucleic acid, and Mg^2＋ and K^＋ contents under tile action of Ag-antibiotic 702 determined, and the effects of Ag-antibiotic 702 on the cell membrane ergosterol biosynthesis and uhramicrostructure observed. The results showed that the active products of Ag-antibiotic 702 had stronger inhibition effect on 13 test pathogens, among which, Sclerotinia sclerotiorum was most sensitive, with the ECs0being 0.23 3μg·mL^-1. As compared with the control, the relative electric conductivity of R. solani treated with Ag-antibiotic 702 was increased by 72.2% , the contents of protein, nucleic acid, and Mg^2＋ and K^＋leaked from the R. solani cells were all increased, while the ergosterol content was decreased by 92.0%. The cell membrane outline was not clear, organelles were inhibition of ergosterol biosynthesis action mechanisms of Ag-antihiotic badly damaged, and vacuole appeared. It was suggested that the and the increase of membrane permeability could be the main 702 against pathogenic fungi.

关 键 词：农抗702 抑制作用 水稻纹枯病菌 

分 类 号：S482.2[农业科学—农药学]