肺结核患者Thl7和调节性T细胞在抗结核治疗过程中的变化  

The changes of Thl7 and regulatory T cells in the process of anti-tuberculosis treatment of tuberculo-sis patients

作　　者：李靖[1] 何艳[2] 郑煜煌[2] 刘艳科[1] 尹柯[1] 封文军[1] 杨晓云[1] 刘超群[1] 何芳[1] 屈满英[1] 欧阳成林[1] 

机构地区：[1]长沙市中心医院胸五科,410004 [2]中南大学湘雅二医院感染科艾滋病研究室,长沙410011

出　　处：《中华微生物学和免疫学杂志》2012年第9期813-815,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的观察肺结核患者CD3＋CD4＋IL-17＋T细胞（Thl7细胞）和CD4＋CD25＋Foxp3＋T细胞（调节性T细胞，Tr）的平衡状态及其在6个月抗结核治疗中的变化。方法选取经抗结核治疗的肺结核患者32例，同时选取32例健康志愿者为对照，分别于治疗0、3、6个月采集静脉血，用流式检测Thl7细胞及Tr细胞百分比，并对比分析其相互关系。结果肺结核患者在抗结核治疗前、治疗3月、6个月与正常人外周血的Thl7细胞在CD4＋T细胞中的比例分别为（1．10±0．39）％、（2．50±1．03）％、（3．90±1．34）％、（4．50±1．52）％；Tr细胞在CD4＋T细胞中的比例分别为（9．17±3．26）％、（6．85±2．73）％、（5．46±1．69）％、（4．35±0．86）％。结论结核分枝杆菌感染导致Thl7细胞和Tr细胞的失平衡，而抗结核治疗可能逐渐恢复二者的免疫平衡状态。免疫细胞量的变化有可能成为观察肺结核进展和抗结核疗效的参考指标。Objective To observe the Thl7 and regulatory T cells（Tr） equilibrium state as well as their changes of tuberculosis patients in six-month＇s anti-Tuberculosis treatment. Methods Select thirty- two tuberculosis patients received anti-Tuberculosis treatment while thirty-two healthy Volunteers as controls. Flow cytometry was used to analyze Thl7 and Tr cells in venous blood at the time of pre-therapy, 3th, 6th month. Results The ratio of Thl7 cells in CIM cells in tuberculosis patients and volunteers were （ 1.10±0.39）%, （2.50±1.03）%, （3.90±1.34）%, （4.50±1.52）%, respectively; the ratio of Tr cells were （9.17±3.26）%, （6.85±2.73）%, （5.46±1.69）%, （4.35±0.86）%, respectively. Conclusion Tuberculosis could make Thl7 cells and Tr cells lost their balance, but the immune equilibrium state may grad- ually recover after anti-tuberculosis. The change of the amount of immune cells was likely to be the reference indexes to observe the progress of tuberculosis and the treatment effect of anti-tuberculosis.

关 键 词：肺结核 TH17细胞 调节性T细胞 抗结核治疗 

分 类 号：R521[医药卫生—内科学]