Molecular basis of the acetyltransferase activity of MEC-17 towards α-tubulin  

作　　者：Wenjing Li Chen Zhong Lei Li Bingfa Sun Weiyi Wang Shutong Xu Tianlong Zhang 2Chunguang Wang Lan Bao Jianping Ding 

机构地区：[1]State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [2]State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [3]Institute of Protein Research, Tongji University, Shanghai 200092, China [4]Graduate School of the Chinese Academy of Sciences, 320 Yu- eyang Road, Shanghai 200031, China

出　　处：《Cell Research》2012年第12期1707-1711,共5页细胞研究（英文版）

基　　金：We thank the staff members at the Shanghai Synchrotron Radiation Facility （SSRF）, China for technical support in diffrac- tion data collection. This work was supported by the Ministry of Science and Technology of China （2011CB966301）, the National Natural Science Foundation of China （31221001）, the Chinese Academy of Sciences （KSCX2-EW-Q- 1-03）.

摘　　要：Dear Editor, Adaptation of microtubules to the structural and func- tional complexities involves diverse post-translational modifications of tubulins, including acetylation, tyrosina- tion, detyrosination, polyglycylation, polyglutamylation, palmitoylation, and phosphorylation. In particular, acety- lation of a-tubulin affects the cell motility and plays an important role in neuronal migration and differentiation [1]. Specifically, acetylation of Lys40 of a-tubulin has prominent impacts on polarized protein trafficking and hence protein transport by promoting the binding of dy- nein/dynactin and kinesin-1 motor complexes [2, 3]. Re- cently the Caenorhabditis elegans protein mechanosen- sory abnormality 17 （MEC-17） and its homologues were shown to harbor intrinsic and specific acetyltransferase activity towards Lys40 of ct-tubulin, which is critical in several biological processes such as cilium assembly of ciliated organisms, mechanosensation of C. elegans, and embryonic development ofzebrafish [4, 5].

关 键 词：Α-微管蛋白 乙酰化 酶活性 分子基础 秀丽隐杆线虫 蛋白质运输 生物学过程 翻译后修饰 

分 类 号：Q51[生物学—生物化学] TQ246.38[化学工程—有机化工]