一测多评中待测成分校正和定位的新方法研究  

A new method of calibration and positioning in quantitative analysis of multicomponents by single marker

作　　者：何兵[1] 杨世艳[2] 张燕[3] 

机构地区：[1]泸州医学院药物研究中心,四川泸州646000 [2]泸州医学院附属中医医院,四川泸州646000 [3]泸州医学院附属医院,四川泸州646000

出　　处：《药学学报》2012年第12期1653-1659,共7页Acta Pharmaceutica Sinica

基　　金：泸州市重点科技项目(泸市财企[2010]41号);泸州医学院青年基金资助项目(泸医院[2010]108号)

摘　　要：研究一测多评中待测成分的一种新的校正模式和色谱峰定位方法,以双黄连口服液为研究对象,采用3种校正方法 (多点校正、斜率校正、定量因子校正)建立双黄连口服液中绿原酸与11个活性成分(新绿原酸、隐绿原酸、咖啡酸、连翘酯苷A、野黄芩苷、异绿原酸B、异绿原酸A、异绿原酸C、黄芩苷、连翘苷及汉黄芩苷)之间的相对校正因子,同时采用线性回归法定位色谱峰,实现只用一个对照品测定双黄连口服液中12个成分的含量。结果表明在一定线性范围内,以3种校正方式所得校正因子计算结果与外标法或线性回归法并无显著性差异,且该法更简便快捷、结果准确可靠、具有良好的重现性。而线性回归法定位色谱峰比文献采用相对保留时间更准确。用斜率和定量因子校正控制中药质量的方法可行且结果准确。This paper aims to establish a new method of calibration and positioning in quantitative analysis of multicomponents by single marker （QAMS）, using Shuanghuanglian oral liquid as the research object. Establishing relative correction factors with reference chlorogenic acid to other 11 active components （neochlorogenic acid, cryptochlorogenic acid, cafferic acid, forsythoside A, scutellarin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, baicalin and phillyrin wogonoside） in Shuanghuanglian oral liquid by 3 correction methods （multipoint correction, slope correction and quantitative factor correction）. At the same time chromatographic peak was positioned by linear regression method. Only one standard uas used to determine the content of 12 components in Shuanghuanglian oral liquid, in stead of needing too many reference substance in quality control. The results showed that within the linear ranges, no significant differences were found in the quantitative results of 12 active constituents in 3 batches of Shuanghuanglian oral liquid determined by 3 correction methods and external standard method （ESM） or standard curve method （SCM）. And this method is simpler and quicker than literature methods. The results were accurate and reliable, and had good reproducibility. While the positioning chromatographic peaks by linear regression method was more accurate than relative retention time in literature. The slope and the quantitative factor correction controlling the quality of Chinese traditional medicine is feasible and accurate.

关 键 词：相对校正因子 多点校正 斜率校正 定量因子校正 双黄连口服液 

分 类 号：R917[医药卫生—药物分析学]