采用在体单向肠灌流模型研究葛根芩连汤不同配伍组主要指标成分的肠吸收特性  

Intestinal absorption of different combinations of active compounds from Gegenqinlian decoction by rat single pass intestinal perfusion in situ

作　　者：安叡[1] 张华[1] 张艺竹[1] 徐冉驰[1] 王新宏[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《药学学报》2012年第12期1696-1702,共7页Acta Pharmaceutica Sinica

基　　金：上海市自然科学基金面上项目(12ZR1431500);上海市教育委员会科研创新面上项目(11YZ69);高等学校博士点专项科研基金(20093107110011)

摘　　要：研究葛根芩连汤不同配伍组中主要指标成分在大鼠肠道的在体吸收情况。采用改良大鼠在体单向肠灌流模型,通过颈静脉插管补充血液、肠系膜静脉插管采集血样,同时收集灌流流出液,以高效液相色谱串联质谱(LC-MS/MS)测定样品中葛根素、大豆苷、甘草苷、黄芩苷、汉黄芩苷、药根碱、小檗碱和巴马汀含量,计算其通透率。结果表明,甘草可促进君药葛根中葛根素和大豆苷的吸收,同时葛根也可促进甘草苷的吸收。黄芩苷与汉黄芩苷在全方及不同配伍组的吸收情况表明,葛根和甘草可促进黄芩苷的吸收,且葛根的作用强于甘草;葛根同样可促进汉黄芩苷的吸收,但甘草对其则为抑制作用。葛根与甘草均可促进黄连有效成分药根碱、小檗碱和巴马汀的吸收。The aim is to study the intestinal absorption of different combinations of active compounds out of Gegenqinlian decoction. Rat single pass intestinal perfusion model with jugular vein cannulated was used. Samples were obtained continuously from the outlet perfusate and the mesenteric vein. The levels of puerarin, daidzin, liquilitin, baicalin, wogonoside, jatrorrhizine, berberine and palmatine were determined by LC-MS/MS and their permeability coefficients were calculated. The results showed that Glycyrrhiza could promote the absorption of the active ingredients in Pueraria which is the monarch herb; meanwhile, Pueraria also played a role in promoting the absorption of liquilitin. Based on the Gegenqinlian decoction and the different combinations experiments, the results concerning the absorption of baicalin and wogonoside were as follows. For baicalin, Pueraria and Glycyrrhiza could promote its absorption and the effect of Pueraria was more obvious. For wogonoside, Pueraria could also promote its absorption, while Glycyrrhiza played a opposite role. Pueraria and Glycyrrhiza both played a part in promoting the absorption ofjateorhizine, berberine and palmatine, the effective compounds in Coptis.

关 键 词：葛根芩连汤 在体单向肠灌流模型 肠吸收 

分 类 号：R943[医药卫生—药剂学]