地塞米松对IL-13诱导的大鼠鼻黏膜mCLCA3和Muc5ac表达的影响  

Effects of dexamethasone on IL-13-induced mCLCA3 and Muc5ac expressions in nasal mucosa of rats

作　　者：吴文丽[1] 刘志[1] 仲莉梅[1] 崔鹏程[1] 高鹏飞[1] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉头颈外科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2012年第12期1258-1260,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：陕西省社发攻关项目(2011K12-37)

摘　　要：目的:研究地塞米松对白细胞介素IL-13诱导的大鼠鼻黏膜mCLCA3和黏蛋白Muc5ac表达的影响,探讨其对变应性鼻炎黏液分泌的影响。方法:30只SD大鼠随机分成对照组、IL-13组、地塞米松组,用逆转录聚合酶链反应法(RT-PCR)和免疫组化法分别检测鼻黏膜mCLCA3 mRNA和Muc5ac蛋白的表达。结果:对照组大鼠鼻黏膜中mCLCA3mRNA表达为0.000±0.000,IL-13组为0.319±0.121,两组比较差异有统计学意义(P<0.05);地塞米松组为0.144±0.105,IL-13组为0.319±0.121,两组比较差异有统计学意义(P<0.05);对照组大鼠鼻黏膜中Muc5ac蛋白的表达(0.300±0.145)与IL-13组(1.389±0.499)比较,差异有统计学意义(P<0.05);地塞米松组Muc5ac蛋白的表达(0.901±0.390)较IL-13组(1.389±0.499)明显减少(P<0.05)。结论:IL-13能够上调mCLCA3 mRNA和Muc5ac在鼻黏膜的表达,地塞米松可下调mCLCA3 mRNA和黏蛋白Muc5ac表达。AIM： To study the effects of dexamethasone on the IL-13-induced mCLCA3 and MucSac expressions in nasal mucosa of rats, and the role in mucus secretion of allergic rhinitis. METHODS： Thirty SD rats were randomly divided into control group, IL-13 group and dexamethasone group. Expressions of mCLCA3 mRNA and Muc5ac protein in nasal mucosa were detected by RT-PCR and immunohistochemistry, respectively. RESULTS： No mCLCA3 mRNA expression in the nasal mucosa was detected in the control group, while it was 0.319 ±0.121 in the IL- 13 group （P〈0.05 vs control group） and 0. 144 ±0. 105 in the dexamethasone group （P 〈 0.05 vs IL-13 group）. The expression of MucSac protein increased in the IL-13 group （1.389 ±0.499） as compared with the control group （0.300 ± 0.14.5, P〈0.05）. After treatment with dexamethasone, the expression of Muc5ac protein was notably lower （0.901 ±0.390） than that in IL-13 group （ P 〈 0.05）. CONCLUSION： IL-13 up-regulates the expressions of mCLCA3 mRNA and MucSac protein in nasal mucosa, which may play a pivotal role in the mucus overproduction of nasal mucosa. Dexamethasone substantially down-regulates the expressions of mCLCA3 mRNA and Muc5ac protein, which may have an inhibiting effect on mucus overproduction of nasal mucosa.

关 键 词：地塞米松 白细胞介素-13 氯通道 黏蛋白 变应性鼻炎 

分 类 号：R393.12[医药卫生—基础医学] R765.21