乳酸乳球菌同时发送外源性蛋白质/DNA到哺乳动物细胞  

Co-delivery of exogenous protein and DNA into mammalian cells with Lactococcus lactis

作　　者：李轶杰[1] 刘欢欢[1] 张富春[1] 

机构地区：[1]新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《细胞与分子免疫学杂志》2012年第12期1328-1330,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30860265);新疆生物资源基因工程重点实验室基金(XJDX0201-2005-02)

摘　　要：目的:建立乳酸乳球菌同时发送外源蛋白/DNA到哺乳动物细胞的系统。方法:分别将红色荧光蛋白基因、绿色荧光蛋白真核表达框整合于乳酸菌穿梭载体pMG36e中相关部位。重组质粒电转乳酸乳球菌后,经甘氨酸消弱乳酸乳球菌细胞膜后与哺乳动物细胞293T细胞共培养。结果:红色和绿色荧光蛋白均能在293T细胞表达。结论:借助乳酸乳球菌成功实现同时发送蛋白质/DNA到哺乳动物细胞。AIM： To develop a co-delivery system of exogenous protein and DNA into mammalian cells using Lactococcus lactis （ L. lactis）. METHODS： We constructed E. coli-L, lactis shuttle plasmid, pMG36e-RFP/eGFP, containing a L. lactis expression cassette with the cDNA of red fluorescent protein （RFP） gene and a eukaryotic expression cassette with the cDNA of enhanced green fluorescent protein （eGFP）, then electrotransformated it into L. lactis cells, and co-cultured with 293T cells to evaluate the role of the system in the improvement of gene delivering efficiency after L. lactis cells were pretreated with glycine. RFP and GFP expressed in 293T cells was monitored by fluorescence microscopy. RESULTS： Fluorescence microscopy revealed that the RFP and GFP was expressed in 293T cells. CONCLUSION： The co-delivery system of protein and DNA into mammalian cells using L. lactis was constructed successfully.

关 键 词：乳酸乳球菌 双表达 共发送 

分 类 号：Q785[生物学—分子生物学]